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http://ir.lib.ncu.edu.tw/handle/987654321/50743
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| Title: | Green Tea Epigallocatechin Gallate Inhibits Insulin Stimulation of Adipocyte Glucose Uptake via the 67-Kilodalton Laminin Receptor and AMP-Activated Protein Kinase Pathways |
| Authors: | Hsieh,CF;Tsuei,YW;Liu,CW;Kao,CC;Shih,LJ;Ho,LT;Wu,LY;Wu,CP;Tsai,PH;Chang,HH;Ku,HC;Kao,YH |
| Contributors: | 生命科學系 |
| Keywords: | CATECHIN-POLYPHENOLS;TRANSPORTER GLUT1;CELLS;EGCG;MODULATION;MECHANISMS;CAFFEINE;RESISTIN |
| Date: | 2010 |
| Issue Date: | 2012-03-27 18:09:10 (UTC+8) |
| Publisher: | 國立中央大學 |
| Abstract: | Insulin and (-)-epigallocatechin gallate (EGCG) are reported to regulate obesity and fat accumulation, respectively. This study investigated the pathways involved in EGCG modulation of insulin-stimulated glucose uptake in 3T3-L1 and C3H10T1/2 adipocytes. EGCG inhibited insulin stimulation of adipocyte glucose uptake in a dose- and time-dependent manner. The concentration of EGCG that decreased insulin-stimulated glucose uptake by 50-60% was approximately 5-10 mu M for a period of 2h. At 10 mu M, EGCG and gallic acid were more effective than (-)-epicatechin, (-)-epigallocatechin, and (-)-epicatechin 3-gallate. We identified the EGCG receptor [also known as the 67-kDa laminin receptor (67LR)] in fat cells and extended the findings for this study to clarify whether EGCG-induced changes in insulin-stimulated glucose uptake in adipocytes could be mediated through the 67LR. Pretreatment of adipocytes with a 67LR antibody, but not normal rabbit immunoglobulin, prevented the effects of EGCG on insulin-increased glucose uptake. This suggests that the 67LR mediates the effect of EGCG on insulin-stimulated glucose uptake in adipocytes. Moreover, pretreatment with an AMP-activated protein kinase (AMPK) inhibitor, such as compound C, but not with a glutathione (GSH) activator, such as N-acetyl-L-cysteine (NAC), blocked the antiinsulin effect of EGCG on adipocyte glucose uptake. These data suggest that EGCG exerts its anti-insulin action on adipocyte glucose uptake via the AMPK, but not the GSH, pathway. The results of this study possibly support that EGCG mediates fat content. |
| Relation: | PLANTA MEDICA |
| Appears in Collections: | [生命科學系] 期刊論文
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