Schizosaccharomyces pombe Possesses Two Paralogous Valyl-tRNA Synthetase Genes of Mitochondrial Origin

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题名:	Schizosaccharomyces pombe Possesses Two Paralogous Valyl-tRNA Synthetase Genes of Mitochondrial Origin
作者:	Chiu,WC;Chang,CP;Wen,WL;Wang,SW;Wang,CC
贡献者:	生命科學系
关键词:	METHIONYL-TRANSFER-RNA;SACCHAROMYCES-CEREVISIAE;BINDING DOMAIN;YEAST;TRANSLATION;AMINOACYLATION;RECOGNITION;REDUNDANCY;MECHANISM;COFACTOR
日期:	2010
上传时间:	2012-03-27 18:09:40 (UTC+8)
出版者:	國立中央大學
摘要:	Previous studies showed that VAS1 of Saccharomyces cerevisiae encodes both cytosolic and mitochondrial forms of valyl-tRNA synthetase (ValRS) through alternative initiation of translation. We show herein that except for Schizosaccharomyces pombe, all yeast species studied contained a single ValRS gene encoding both forms, and all of the mature protein forms deduced from those genes possessed an N-terminal appended domain (Ad) that was absent from their bacterial relatives. In contrast, S. pombe contained two distinct nuclear ValRS genes, one encoding the mitochondrial form and the other its cytosolic counterpart. Although the cytosolic form closely resembles other yeast ValRS sequences (similar to 60% identity), the mitochondrial form exhibits significant divergence from others (similar to 35% identity). Both genes are active and essential for the survival of the yeast. Most conspicuously, the mitochondrial form lacks the characteristic Ad. A phylogenetic analysis further suggested that both forms of S. pombe ValRS are of mitochondrial origin, and the mitochondrial form is ancestral to the cytoplasmic form.
關聯:	MOLECULAR BIOLOGY AND EVOLUTION
显示于类别:	[生命科學系] 期刊論文

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