English  |  正體中文  |  简体中文  |  Items with full text/Total items : 65421/65421 (100%)
Visitors : 22281681      Online Users : 186
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/50784


    Title: A simple method using Pyrosequencing (TM) to identify de novo SNPs in pooled DNA samples
    Authors: Lin,YS;Liu,FGR;Wang,TY;Pan,CT;Chang,WT;Li,WH
    Contributors: 生命科學系
    Keywords: ALLELE FREQUENCY ESTIMATION;SINGLE NUCLEOTIDE POLYMORPHISMS;COMPLEX HUMAN-DISEASES;CASE-CONTROL DESIGNS;LINKAGE DISEQUILIBRIUM;MUTATION DETECTION;SEQUENCING METHOD;RELATIVE POWER;TECHNOLOGY;QUANTIFICATION
    Date: 2011
    Issue Date: 2012-03-27 18:10:07 (UTC+8)
    Publisher: 國立中央大學
    Abstract: A practical way to reduce the cost of surveying single-nucleotide polymorphism (SNP) in a large number of individuals is to measure the allele frequencies in pooled DNA samples. Pyrosequencing (TM) has been frequently used for this application because signals generated by this approach are proportional to the amount of DNA templates. The Pyrosequencing (TM) pyrogram is determined by the dispensing order of dNTPs, which is usually designed based on the known SNPs to avoid asynchronistic extensions of heterozygous sequences. Therefore, utilizing the pyrogram signals to identify de novo SNPs in DNA pools has never been undertook. Here, in this study we developed an algorithm to address this issue. With the sequence and pyrogram of the wild-type allele known in advance, we could use the pyrogram obtained from the pooled DNA sample to predict the sequence of the unknown mutant allele (de novo SNP) and estimate its allele frequency. Both computational simulation and experimental Pyrosequencing(TM) test results suggested that our method performs well. The web interface of our method is available at http://life.nctu.edu.tw/similar to yslin/PSM/.
    Relation: NUCLEIC ACIDS RESEARCH
    Appears in Collections:[生命科學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML370View/Open


    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback  - 隱私權政策聲明