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    請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/51075


    題名: N(epsilon)-Lysine Acetylation of a Bacterial Transcription Factor Inhibits Its DNA-Binding Activity
    作者: Thao,S;Chen,CS;Zhu,H;Escalante-Semerena,JC
    貢獻者: 系統生物與生物資訊研究所
    關鍵詞: ESCHERICHIA-COLI K-12;ERWINIA-AMYLOVORA;CAPSULE SYNTHESIS;RCS PHOSPHORELAY;COA SYNTHETASE;COLANIC ACID;RECOMBINANT PROTEINS;EXPRESSION;REGULATOR;SYSTEM
    日期: 2010
    上傳時間: 2012-03-27 18:20:42 (UTC+8)
    出版者: 國立中央大學
    摘要: Evidence suggesting that eukaryotes and archaea use reversible N(epsilon)-lysine (N(epsilon)-Lys) acetylation to modulate gene expression has been reported, but evidence for bacterial use of N(epsilon)-Lys acetylation for this purpose is lacking. Here, we report data in support of the notion that bacteria can control gene expression by modulating the acetylation state of transcription factors (TFs). We screened the E. coli proteome for substrates of the bacterial Gcn5-like protein acetyltransferase (Pat). Pat acetylated four TFs, including the RcsB global regulatory protein, which controls cell division, and capsule and flagellum biosynthesis in many bacteria. Pat acetylated residue Lys180 of RcsB, and the NAD+-dependent Sir2 (sirtuin)-like protein deacetylase (CobB) deacetylated acetylated RcsB (RcsB(Ac)), demonstrating that N(epsilon)-Lys acetylation of RcsB is reversible. Analysis of RcsB(Ac) and variant RcsB proteins carrying substitutions at Lys180 provided biochemical and physiological evidence implicating Lys180 as a critical residue for RcsB DNA-binding activity. These findings further the likelihood that reversible N(epsilon)-Lys acetylation of transcription factors is a mode of regulation of gene expression used by all cells.
    關聯: PLOS ONE
    顯示於類別:[系統生物與生物資訊研究所] 期刊論文

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