中大機構典藏-NCU Institutional Repository-提供博碩士論文、考古題、期刊論文、研究計畫等下載:Item 987654321/51081
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 70548/70548 (100%)
造访人次 : 23136615      在线人数 : 371
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/51081


    题名: A chemical probe selectively inhibits G9a and GLP methyltransferase activity in cells
    作者: Vedadi,M;Barsyte-Lovejoy,D;Liu,F;Rival-Gervier,S;Allali-Hassani,A;Labrie,V;Wigle,TJ;DiMaggio,PA;Wasney,GA;Siarheyeva,A;Dong,AP;Tempel,W;Wang,SC;Chen,X;Chau,I;Mangano,TJ;Huang,XP;Simpson,CD;Pattenden,SG;Norris,JL;Kireev,DB;Tripathy,A;Edwards,A;Roth,BL;Janzen,WP;Garcia,BA;Petronis,A;Ellis,J;Brown,PJ;Frye,SV;Arrowsmith,CH;Jin,J
    贡献者: 系統生物與生物資訊研究所
    关键词: PLURIPOTENT STEM-CELLS;HISTONE METHYLTRANSFERASES;DNA METHYLATION;LYSINE-9 METHYLATION;CHROMATIN-STRUCTURE;CANCER-CELLS;P53;TRANSCRIPTION;REPRESSION;COMPLEXES
    日期: 2011
    上传时间: 2012-03-27 18:20:52 (UTC+8)
    出版者: 國立中央大學
    摘要: Protein lysine methyltransferases G9a and GLP modulate the transcriptional repression of a variety of genes via dimethylation of Lys9 on histone H3 (H3K9me2) as well as dimethylation of non-histone targets. Here we report the discovery of UNC0638, an inhibitor of G9a and GLP with excellent potency and selectivity over a wide range of epigenetic and non-epigenetic targets. UNC0638 treatment of a variety of cell lines resulted in lower global H3K9me2 levels, equivalent to levels observed for small hairpin RNA knockdown of G9a and GLP with the functional potency of UNC0638 being well separated from its toxicity. UNC0638 markedly reduced the clonogenicity of MCF7 cells, reduced the abundance of H3K9me2 marks at promoters of known G9a-regulated endogenous genes and disproportionately affected several genomic loci encoding microRNAs. In mouse embryonic stem cells, UNC0638 reactivated G9a-silenced genes and a retroviral reporter gene in a concentration-dependent manner without promoting differentiation.
    關聯: NATURE CHEMICAL BIOLOGY
    显示于类别:[系統生物與生物資訊研究所] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML597检视/开启


    在NCUIR中所有的数据项都受到原著作权保护.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈  - 隱私權政策聲明