English  |  正體中文  |  简体中文  |  Items with full text/Total items : 69937/69937 (100%)
Visitors : 23089109      Online Users : 725
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/52105


    Title: Surface plasmon resonance biosensor with high anti-fouling ability for the detection of cardiac marker troponin T
    Authors: Liu,JT;Chen,CJ;Ikoma,T;Yoshioka,T;Cross,JS;Chang,SJ;Tsai,JZ;Tanaka,J
    Contributors: 電機工程學系
    Keywords: SELF-ASSEMBLED MONOLAYERS;PROTEIN ADSORPTION;MOLECULAR-CONFORMATION;SPR IMMUNOSENSOR;MODEL SYSTEM;GOLD;RESIST;ACID;SEPARATION;BLOOD
    Date: 2011
    Issue Date: 2012-03-28 10:15:34 (UTC+8)
    Publisher: 國立中央大學
    Abstract: Designing a surface recognition layer with high anti-fouling ability, high affinity, and high specificity is an important issue to produce high sensitivity biosensing transducers. In this study, a self-assembled monolayer (SAM) consisting of a homogeneous mixture of oligo(ethylene glycol) (OEG)-terminated alkanethiolate and mercaptohexadecanoic acid (MHDA) on Au was employed for immobilizing troponin T antibody and applied in detecting cardiac troponin T by using surface plasmon resonance (SPR). The mixed SAM showed no phase segregation and exhibited human serum albumin resistance, particularly with an antibody-immobilized surface. X-ray photoemission spectra revealed that the chemical composition ratio of OEG to the mixed SAM was 69% and the OEG packing density was 82%. The specific binding of troponin Ton the designed surface indicated a good linear correlation (R = 0.991, P < 0.0009) at concentrations lower than 50 mu g mL(-1) with the limit of detection of 100 ng mL(-1) using a SPR measuring instrument. It is concluded that the mixed SAM functions as designed since it has high detection capability. high accuracy and reproducibility, as well as shows strong potential to be applied in rapid clinical diagnosis for label-free detection within 2 min. (C) 2011 Elsevier B.V. All rights reserved.
    Relation: ANALYTICA CHIMICA ACTA
    Appears in Collections:[電機工程學系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML251View/Open


    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback  - 隱私權政策聲明