利用Bacillus sp. 分解纖維素進行兩階段式發酵生產特用化學品;Study of converting cellulose to Specialty Chemical on the two-step fermentation by Bacillus sp.

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题名:	利用Bacillus sp. 分解纖維素進行兩階段式發酵生產特用化學品;Study of converting cellulose to Specialty Chemical on the two-step fermentation by Bacillus sp.
作者:	林文仕;Lin,Wen-shi
贡献者:	化學工程與材料工程研究所
关键词:	芽孢桿菌;土麴黴;衣康酸;纖維水解酵素;纖維素;Aspergillus terreus;Bacillus sp.;cellulose;cellulase;Itaconic acid
日期:	2012-08-15
上传时间:	2012-09-11 18:13:00 (UTC+8)
出版者:	國立中央大學
摘要:	纖維素為地球上龐大的可利用的再生能源，如果能將農業廢棄物中的纖維素藉由纖維水解酵素轉換為葡萄糖並且供應後續菌種發酵利用來生產產物，如此不僅有效率的利用農業廢棄物並且能降低操作成本，提高產能。本論文所使用的菌種Bacillus sp.經由實驗尋找得到纖維水解酵素活性最高的pH值為pH5；最高的酵素活性反應溫度為40℃。而在pH4.8時能停止菌體的生長，並且酵素保有活性，發酵液中的還原醣濃度也會開始累積，而有進行pH值調控的還原醣累積量為1.501g/L比沒有調控的控制組的還原醣累積量0.231g/L高了6.5倍。而經過還原糖累積後的發酵液用來培養Aspergillus terreus則可以提高菌體的重量，菌重是控制組的1.68倍但是在產物衣康酸的生產方面只有控制組的47.7%，所以要再尋找適合衣康酸生產的條件才會有更好的纖維素轉化為衣康酸的效果。累積完的還原醣經由HPLC及GPC分析後認定為纖維寡醣，所以可能必須要再尋找能生產β-葡萄糖苷酵素的菌種將反應後的纖維寡醣再進一步轉換成葡萄糖，才能更有效的被大部分菌種利用來生產所想要的產物。Cellulose, an abundant and renewable energy resource, the cellulose in agricultural waste can be conversion by cellulase for glucose，and glucose can supply the second-step fermentation use to produce products. It will not only efficient use of agricultural waste and can reduce operating costs.The strain used in this study was Bacillus sp. and Aspergillus terreus. The cellulase activity of Bacillus sp. was found optimum at pH 5 and temperature 40 ℃. When pH is 4.8, the cell growth was stopped, and the cellulase retained activity, the reducing sugar in the broth was accumulated. The reducing sugar in the broth with pH controlled could be accumulated to 1.501g/L but without pH controlled could only be accumulated to 0.231g/L. Then we use the broth of reducing sugar was accumulated to culture Aspergillus terreus. And the biomass was 1.68 times to the control group but the Itaconic acid was only 47.7% to the control group. We will have to find the condition which is favorable to Produce Itaconic acid.The reducing sugar was analysis with GPC and HPLC. We found that the reducing sugar was cellooligosaccharide. We will have to find the microorganism with the ability of producing β-1,4-glucosidase for conversion cellooligosaccharide to glucose. The glucose will simple to be used by the most microorganism.
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