本論文為，探討1,3-偶極環化加成反應的應用。在第一部份的目標為合成嚴重呼吸道症候群（Severe Acute Respiratory Syndrome，SARS）冠狀病毒或其他病毒的抑制劑。利用一號位置為β位向的疊氮基核醣與不同的末端炔類進行1,3-偶極環化加成反應，得到60個分子進行篩檢。在第二部份為開發新的晶片表面化學方法，將晶片種上炔類後，再利用1,3-偶級環化加成反應將含有疊氮基的各種小分子種在晶片表面上，以利於作各種檢測的工作。 In this thesis, new application of 1,3-dipolar cycloaddition in the synthesis of nucleotides analogue and preparation of biomolecule microarray were developed. In the first section, a nucleotides library with 60 members were created as inhibitors of SARS coronaviruses or another viruses. By using 1, 3-dipolar cycloaddition, different terminal alkynes reacted with ribofuranosyl azide to give the nucleotides library. In the second section , a new methodology was developed to immobilize small organic molecules on glass slide. The azido compounds were implanted on chip surface with alkyne functionality by 1,3-dipolar cycloaddition.
