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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/61488


    Title: 以電泳膠體分離及質譜儀鑑定紅斑性狼瘡(活躍期)血漿中表現差異的蛋白質;Identification of proteins with different expression in plasma from active systemic lupus erythematosus by gel electrophoresis and mass spectrometry
    Authors: 李怡萱;Li,Yi-Syuan
    Contributors: 系統生物與生物資訊研究所
    Keywords: 紅斑性狼瘡;蛋白質體學;膠體電泳;systemic lupus erythematosus;proteomic;electrophoresis
    Date: 2013-08-28
    Issue Date: 2013-10-08 15:13:49 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 紅斑性?瘡是一種具有多種?床病症,由自體免疫?常所導致的自體免疫性疾病,
    屬於風濕病的其中一種。紅斑性?瘡症?包含皮疹、發燒、關節炎、感到疲倦及腎絲球
    炎及?經症?等,紅斑性?瘡症?與其他疾病相似,因此容?誤診。本實驗主要目的為
    尋找台灣紅斑性?瘡與健康人體中?同的蛋白質,可提供具潛?的生物標記分子,應用
    於此疾病之診斷上。國泰綜合醫院過敏免疫科提供血漿樣本,共有19 位病人 (包含18
    位?性與1 位男性) 及12 位健康對照組 (包含9 位?性與3 位男性),病人平均?齡為
    32.1±11.5,健康對照組之平均?齡為41.9±12.2。?用蛋白質體學的方法,固定血漿蛋
    白總?,並以1 維與2 維膠體電泳法將病患及健康對照組的蛋白質進?分?,以影像分
    析將蛋白質表現?進?統計分析,病人與健康對照組的?值比較,選取具差?的蛋白質
    (U-test, p<0.5),從膠體上?下,經過蛋白質水解酵素水解後,?用?種質譜儀 (MALDI
    Q-TOF and ESI-MS/MS) 進?蛋白質體身份鑑定。此實驗中,一共找到15 個蛋白質,9
    個為表現?增加的蛋白質。其中9 個蛋白質是文獻已發表和紅斑性?瘡有關,顯示本方
    法可快速全面檢視表現差?蛋白質。而6 個蛋白質為本研究首次發現與紅斑性?瘡有關,
    顯示本方法可提供其他方法無法測得之差?蛋白質。本研究提供?快速尋找新紅斑性?
    瘡具潛?的生物標記分子的方法,將?也可能可以應用於?用血漿樣品尋找其他疾病的
    潛?蛋白質標記分子。
    Systemic lupus erythematous (SLE) exhibits an aggressive clinical phenotype with
    severe complications and overall poor prognosis. Common initial and chronic complaints
    include fever, joint pains, fatigue and temporary loss of cognitive abilities. These symptoms
    are so similar with other disease, so it is very difficult to diagnose and treat. The goal of this
    study is to identify significant different proteins between SLE patients compared to healthy
    people, the significant proteins may help to diagnose SLE patients. The SLE plasma samples
    which included male and 18 female patients, with average age 32.1±11.5 also diagnosed with
    renal involvement. The SLE plasma samples were collected from Cathay General Hospital,
    Taipei, Taiwan. The protein profile from patients was randomly compared to that from twelve
    healthy controls from 3 male and 9 female with average age at 41.9±12.2. The protein
    concentration in the plasma samples was quantified and the total protein was separated by
    one-dimensional and two-dimensional SDS-polyacrylamide gel electrophoresis. The up- and
    down-regulated proteins compared to healthy plasma (U-test, p < 0.5) were selected and cut
    from the gels and digested with trypsin for enzymatic analysis of protein using mass
    spectrophotometry for identification of proteomic analysis followed by the amino acid
    sequencing with mass spectrometry (MALDI-TOF/TOF and ESI-MS/MS). This experiments
    results a total of 15 proteins found, 9 proteins are up-regulated and 6 proteins are down
    regulated. In total of 15 proteins, nine proteins are published relation with lupus and six
    proteins are new.These new proteins in the SLE patients may contribute to find the new
    potential lupus biomarkers determine the precise role of newly identified SLE-related proteins
    pathogenesis and their function as biomarkers will require further study.
    Appears in Collections:[系統生物與生物資訊研究所] 博碩士論文

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