高數值孔徑顯微物鏡設計; High NA objective lens design in hyperspectralmicroscope system

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/61819

Title:	高數值孔徑顯微物鏡設計;High NA objective lens design in hyperspectralmicroscope system
Authors:	吳思漢;Wu,Sih-han
Contributors:	光電科學與工程學系
Keywords:	鏡頭設計;幾何光學;顯微鏡;物鏡;lens design;high NA;objective lens
Date:	2013-12-02
Issue Date:	2014-02-13 17:43:03 (UTC+8)
Publisher:	國立中央大學
Abstract:	在超光譜顯微術系統中，所需的不只是各項元件的品質要求，在成像端更需要高倍率、高成像品質之顯微物鏡。本論文提出一具有高放大倍率、高數值孔徑以及長工作距離的顯微物鏡設計，目的是將之在超光譜顯微術系統中的擷取影像端達到較高的品質，並且讓系統的可用範圍更廣，不只限制在較小的工作範圍，而是加大整個工作區可用性。而超光譜顯微術系統所量測的是生物樣本的激發螢光，因此本論文提出設計一顯微物鏡，利用油浸的方式，使之入射成像面的角度可達到NA=1.2，而所激發之螢光光譜能透過高數值孔徑的物鏡收集，回到後方成像端。本設計的特點在於高數值孔徑以及長工作距離，工作距離拉長除了上述所說的增加系統可用性外，也能夠使樣本量測的範圍加大，不會侷限在特定的距離內。本論文的設計目標為P-V OPD = 0.125 wave、視場 = 240μm×240μm、working distance(WD) = 1mm以及放大倍率M = 60×。 In the hyperspectal microscope system, not only every element quality, but also request image quality on the image space, so the system needed a microscope objective lens of high magnification and high image quality. This dissertation is discusses about lens design of a microscope objective lens of high magnification, high numerical aperture and long working distance, the purpose is build a hyperspectral microscope system to obtain high quality molecular imaging information, also to expand usability of this system. The purpose of hyperspectal microscope system is to obtain the excited fluorescence of biological sample, theobjective lens design of this system utilizedoil immersion to design this lens, and the fluorescence signal will be collected by the objective lensback to the imaging system. The characteristic of this design is this objective lens has high magnification, high numerical aperture and long working distance. This dissertationwill resultin P-V OPD = 0.125 wave, Fov = 240μm×240μm, working distance = 1mm and magnification = 60×.
Appears in Collections:	[Graduate Institute of Optics and Photonics] Electronic Thesis & Dissertation

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