雙光子螢光超光譜顯微術;Two-Photon Fluorescence Hyperspectral Microscopy

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/61953

Title:	雙光子螢光超光譜顯微術;Two-Photon Fluorescence Hyperspectral Microscopy
Authors:	陳思妤
Contributors:	國立中央大學光電科學與工程學系
Keywords:	光電工程
Date:	2014-03-11
Issue Date:	2014-03-11 13:06:18 (UTC+8)
Publisher:	行政院國家科學委員會
Abstract:	研究期間：10308~10407;Recently, molecular imaging becomes a very important topic of biomedical researches. However, since the molecular composition of bio-tissues is quite complicated and fluorescence usually has a broad bandwidth, the emission spectrum of different molecules may easily overlaps. Therefore, there always exist serious crosstalk problems when using beamsplitters and filters in traditional microscopy to get molecular images and it’s difficult to obtain molecular images with high accuracy. Different from traditional microscopy, hyperspectral microscopy can record both spatial and spectral information simultaneously. Through spectrum analysis, the crosstalk problems can be sufficiently avoided. Based on the hyperspectral microsocpy’s capability for molecular imaging, various kinds of hyperpsectral microscopy have been applied to many biomedical researches, such as the molecular imaging of in vitro cultured cells, biopsied tissues, live tissues, and whole animals. Due to the capability for molecular imaging, hyperspectral microscopy plays an important role in both basic biomedical research and clinical diagnosis. Based on its significance, in this proposal, we plan to develop a laser-scanning two-photon fluorescence hyperspectral microscopy. Combing the optical sectioning power in thick tissue, lower photodamage, and higher penetrability provided by two-photon fluorescence microscopy and hyperspectral microscopy’s capability for molecular imaging, this hyperspectral system can be expected to be applied to in vivo or clinical applications to perform in vivo molecular imaging and to provide more valuable molecular information for biomedical researches and clinical diagnosis.
Relation:	財團法人國家實驗研究院科技政策研究與資訊中心
Appears in Collections:	[Department of Optics and Photonics] Research Project

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