生物除磷反應槽中可分離微生物菌相多樣性的探討; The study of the isolated microbial divisity in the EBPR reactor

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题名:	生物除磷反應槽中可分離微生物菌相多樣性的探討;The study of the isolated microbial divisity in the EBPR reactor
作者:	鄭閔魁;Ming-Kui Cheng
贡献者:	生命科學研究所
关键词:	親源樹;微生物分離法;多樣性;分類學;微生物;活性污泥;生物除磷;去氧核醣核酸;16S rDNA;Proteobacteria;Acinetobacter spp;DNA extract;DGGE;EBPR reactor;clone library;PCR
日期:	2000-07-20
上传时间:	2009-09-22 10:16:11 (UTC+8)
出版者:	國立中央大學圖書館
摘要:	生物除磷反應槽是利用微生物(磷蓄積菌)厭氧釋磷好氧攝磷的程序，進行對廢水中磷去除，因此，微生物在生物除磷反應槽中即成了最重要的除磷角色。而本實驗以傳統微生物分離法對生物除磷反應槽的菌群進行探討，共分離出96株細菌，再利用DGGE (denaturing gradient gel eletrophoroesis)的方式快速篩選成34種不同的菌種，經過16S rDNA定序之後，發現21種不同的近似菌種，以類似Microvirgula aerodenitrificans的菌種最多(約32株)，屬β-subdivision Proteobacteria，因此，該菌種極可能為此反應槽中可分離菌群的優勢菌群。另外，以α-subdivision Proteobacteria所包含的種類最多，大約17種，其他如Gram positive HGC或LGC，及CFB group都是重要發現的近似菌種。進一步與非微生物分離法(Nielsen et al., 2000) 比對，發現了以CFB group中的Flavobacterium ferrugineum為與本實驗唯一相同的近似菌種，其他的菌種都不相同，但也證明了一點，許多菌利用傳統微生物分離法是無法發現的，如果兩者能並行使用，所得的結果會更加完整。 The basic principle of Enhanced biological phosphorous removal (EBPR) process is the anaerobic release and aerobic surplus uptake of phosphorus from wastewater by phosphorous accumulating organisms (PAOs). The existence of a stable PAO community is very important to maintain efficient treatment of phosphate. In this study, the traditional isolation method was used to isolate pure cultures from an anoxic-oxic process. 96 strains were isolated from an efficient phosphorous removal A-O process. These 96 strains were screened using DGGE of 16S rDNA and grouped into 34 groups according to the closeness of bands expressed on DGGE. After 16S rDNA sequencing, 21 distinctively different strains were identified. 32 bacterial strains belonged to theβ-subdivision Proteobacteria, 17 strains to theα-subdivision Proteobacteria. Moreover, members of Gram positive HGC, and Cytophaga- Flexibacter- Bacteroids. By further comparison with Gram positive LGC phylums were also isolated. 16S rDNA clone library study (Nielsen et al., 2000), only bacterial strains affiliated with Flavobacterium ferrugineum of the CFB group was common group found. This finding suggests that a polyphasic approach is useful in the determination of microbial community.
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