探討植物DEAD-box RNA helicase 36 (RH36) 如何參與rRNA biogenesis 之分子機制;Study Plant Dead-Box RNA Helicase 36 (RH36) in rRNA Biogenesis

NCU Institutional Repository > 生醫理工學院 > 生命科學系 > 研究計畫 > Item 987654321/62505

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/62505

题名:	探討植物DEAD-box RNA helicase 36 (RH36) 如何參與rRNA biogenesis 之分子機制;Study Plant Dead-Box RNA Helicase 36 (RH36) in rRNA Biogenesis
作者:	陸重安
贡献者:	國立中央大學生命科學系
关键词:	生物科學
日期:	2012-12-01
上传时间:	2014-03-17 11:34:22 (UTC+8)
出版者:	行政院國家科學委員會
摘要:	研究期間：10108~10207;RNA molecules play important roles not only as information carriers, but also in structural compartment and catalytic function. Overall, RNAs are synthesized and processed in nucleus and transferred into to cytoplasm. Base on specific functions of RNAs, RNA molecules can be long single strands or short strands folded into specific tertiary structures. It is known that a variety of proteins are required to assure the correct folding and to maintain or modify specific secondary or tertiary structures of RNAs. Among of these proteins, the DEAD box RNA helicase families are involved in nuclear transcription, pre-mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and gene expression. Although DEAD box RNA helicases are believed as important regulators for RNA molecules, the specific biological roles of individual RNA helicases are still unclear. Yeast is a good host system to study the DEAD box RNA helicases, many of valuable studies have been reported previously. However, most high eukaryotic organisms are multi-cellular containing more complicated metabolic systems than yeast. Thus, only study in yeast sometimes led to unrespectable biological functions of DEAD box RNA helicases in high eukaryotic organisms. In order to identify the specific role of a DEAD box RNA helicase, RH36, both in Arabidopsis and rice, we propose to use genetic, molecular biology and cell biology approaches to address. We reported that a rice and Arabidopsis DEAD box RNA helicase 36 is function in rRNA biogenesis. Moreover, we demonstrated that the RH36 is involved in the female gametophyte development in plants. Further investigations are needed to elucidate the molecular mechanism of RH36 in plant rRNA biogenesis and how to affect the plant female gematogenesis. Therefore, three major subjects are proposed. First, to elucidate how RH36 is involved in plant rRNA biogenesis, inducible overexpression and RNAi of OsRH36 and AtRH36 are applied. Second, we will identify the specific helicase activity and its substrate specificity. Finally, we will analyze RH36 interaction proteins and demonstrate their biological functions. This project, together with in vitro helicase activity assay and transgenic plant system, will provide biochemical and the biological function of RH36 in plants. The value of this project is to directly demonstrate the role of RH36 in plant female development. The potential agricultural value of this project is to expand applications of DEAD box RNA helicase for seed sterility and stress tolerance in rice.
關聯:	財團法人國家實驗研究院科技政策研究與資訊中心
显示于类别:	[生命科學系] 研究計畫

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	420	检视/开启

在NCUIR中所有的数据项都受到原著作权保护.

社群 sharing

数据加载中.....