English  |  正體中文  |  简体中文  |  Items with full text/Total items : 78852/78852 (100%)
Visitors : 37657988      Online Users : 340
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/62527

    Title: 環境逆境下水稻熱休克轉錄因子差異性調控機制分析;Analysis for the Mechanism of Differential Regulation of Rice Heat Shock Transcription Factors under Environmental Stress
    Authors: 葉靖輝;吳少傑
    Contributors: 國立中央大學生命科學研究所
    Keywords: 農藝;生物技術(農)
    Date: 2013-12-01
    Issue Date: 2014-03-17 11:49:15 (UTC+8)
    Publisher: 行政院國家科學委員會
    Abstract: 研究期間:10208~10307;Our previous studies have shown that the expression profiles of rice class I small heat shock protein (sHSP-CIs) genes were diversified in response to heat and azetidine (AZC) stresses. We have found that an AZRE (Azetidine responsive element; GTCCTGGACG), localized on the promoter regions of Oshsp17.3 and Oshsp18.0 genes, was associated with OshsfA4b and heat shock elements (HSEs) to regulate heat shock response and AZC-induced cytosolic protein response. However, OshsfA4b and AZRE did not involve in the induction of Oshsp16.9A gene under heat stress. In this three-year project, our major goal is to further study the differential regulatory mechanism for heat shock factors (HSFs) on the induction of sHSP-CIs genes. Firstly, using the expression level of Oshsp17.3::GUS as an indicator, we are trying to analyze the effects of AZRE and HSEs on the HSF-regulated expression of sHSP-CI genes. Secondly, we plan to screen and characterize other rice HSFs and Arabidopsis HSFs specific for AZC-induced expression of sHSP-CI genes. Thirdly, we plan to introduce AZRE and HSE into rice genome and establish transgenic rice plants, which are used to study the differential regulation of HSFs. Fourthly, we try to use EMSA (Electrophoretic Mobility Shift Assay) technique to analyze the interaction between specific HSFs and AZRE and HSE. Finally, we plan to compare tunicamycin-induced UPR (unfolded protein response) with AZC-induced CPR and UPR.
    Relation: 財團法人國家實驗研究院科技政策研究與資訊中心
    Appears in Collections:[生命科學研究所 ] 研究計畫

    Files in This Item:

    File Description SizeFormat

    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 隱私權政策聲明