本研究主要為改良斷層相位顯微鏡 (Tomographic Phase Microscopy, TPM)，其做法為在新架構中增設反射鏡及離軸全像架構，在保留傳統TPM功能外，亦同時降低系統複雜度以及掃描時間。本系統利用離軸數位全像概念先獲取樣本不同方向的相位投影，再利用相位展開 (Phase Unwrapping, PhU)演算法，將包裹相位展開成連續相位分佈，最後透過濾波反投影 (Filtered-Back Projection, FBP)演算法，重建物體的三維折射率分佈。 本研究可分為模擬以及實驗兩部分。在模擬方面，主要將各種影像還原演算法建立於重建流程中，用於處理實驗所擷取的原始數據；在實驗部份，先是建構了鏡像輔助TPM系統，而後利用已知粒徑與折射率的小球作為樣本並取得小球的三維折射率分佈來驗證新架構與演算法，亦將本系統應用於細胞三維折射率分佈的量測。最後比較半角度與全角度範圍的小球重建影像相似度。; The main purpose of this study is to improve tomographic phase microscopy (TPM). The method is to increase mirror and off-axis holographic structure in the novel system. Not only saving functions of the traditional TPM, but also reducing system complexity and scanning time. The first, the system uses the concept of off-axis digital holography to obtain phase projections of sample in different directions, and the wrapped phase will be unwrapped to successive phase distribution by phase unwrapping algorithm. Finally, the three-dimensional refractive index distribution of the reconstructed object by filtered backprojection (FBP)algorithm. There are simulation and experiment in the study. In the simulation, the various algorithms about image reconstruction will be established in the reconstruction process for processing the raw data captured by the experiment. In the experiment, the first is the construction of mirror-assisted TPM system. Then using beads of the known diameter and refractive index as a sample to obtain three-dimensional refractive index distribution of the beads for verifying the novel system and algorithms. The system will also be applied to measure the three-dimensional refractive index distribution of cell.