在海洋工業、食品工業與生醫材料中，生物積垢(biofouling)是個棘手的問題。而許多抗生物沾黏(anti-biofouling)官能基已被發現可降低生物沾黏之形成，其中包含了雙離子性材料(zwitterionic materials)，然而雙離子性官能基本身極為親水的特性，使得該材料於疏水性表面上改質極具挑戰。一般而言，疏水材料表面之改質多仰賴化學接枝法，但此種改質方法具有費時、改質均勻性差、製程難以放大等問題。為解決上述問題，我們合成帶有雙離子基團之兩性無規則共聚高分子(amphiphatic random co-polymers)，再利用物理批覆之法將其塗佈於疏水薄膜表面，並調控共聚物中羧基甜菜鹼(carboxybetaine)與丙烯酸十八酯(octadecyl acrylate)之比例。當聚丙烯薄膜表面塗佈兩性雙離子性共聚高分子後，當高分子中雙離子性基團含量增加，薄膜表面會顯得更為親水，然而高分子於膜表面之披覆穩定度也會隨之下降。此外我們也發現了降低生物沾黏形成的最佳高分子組成，當聚丙烯膜表面披覆之兩性雙離子性共聚高分子中雙離子性基團含量為26 %時，該薄膜可大幅降低人類血球、大腸桿菌與HT-1080細胞的貼附。在本研究中，我們對於以物理披覆方式將兩性抗沾黏之高分子塗佈於疏水表面上之方法提供了一些想法。;Biofouling is an essentially critical problem on marine industry, food industry and biomaterials. Anti-biofouling functional groups have been investigated to reduce biofouling formation; zwitterionic materials are one of the effective functional groups. It is a challenge to modify the zwitterionic functional groups on a hydrophobic surface because of the high hydrophilicity of zwitterionic materials. In general, chemical grafting method is usually used, but the grafting process is time-consuming and the uniformity of chemical grafting is not easily controlled in a larger scale. To solve the above mentioned problems, we synthesized the amphiphatic random co-polymers containing of zwitterionic moiety to coat on the hydrophobic membrane physically. We controlled the various ratios of carboxybetaine and octadecyl acrylate in copolymers. After coating the zwitterionic copolymers on polypropylene membrane surface, the hydrophilicity of membrane increases along with the zwitterionic content increases, but the coating stability decreases. We found there is an optimal window for reducing the macromoleular fouling formation. While we used the copolymer containing of 26% zwitterionic moiety to coat on membrane, the biofoulings of human blood cells, E.coli and HT-1080 cells reduce significantly. We provide guidance on physical coating of amphiphatic antifouling copolymer on hydrophobic surface.
