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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/6500

    Title: 以高效液相層析儀搭被螢光偵檢器檢測水樣中Xanthene Dye的含量;Determination of Xanthene Dye in aqueous sample by HPLC-FLD
    Authors: 江宗霖;Tsung-ling CHIANG
    Contributors: 化學研究所
    Keywords: 固相萃取法;高效液相層析儀;羅丹明染料;SPE;rhodamine dye;HPLC
    Date: 2009-06-18
    Issue Date: 2009-09-22 10:20:38 (UTC+8)
    Publisher: 國立中央大學圖書館
    Abstract: 羅丹明B和羅丹明6G皆屬於Xanthene Dyes,除了被當作顏色添加劑而廣泛的使用在化妝品、食品、藥物之中,也被當作著色劑而常被使用在紡織和塑膠工業上。雖然這類染料被大量使用在不同的領域之中,但已經有許多研究指出這些染料會對皮膚、眼睛和呼吸道造成刺激和傷害,而且Xanthene Dyes的致癌性、生殖上和發育上的相關毒性皆已經被證實。   本研究利用高效液相層析儀搭配螢光偵檢器(High Performance Liquid Chromatography-Fluorescence Detector,簡稱HPLC-FLD)發展出一套簡易的方法來檢測台灣環境水樣中羅丹明B和羅丹明6G的含量。最佳的分離條件為使用Waters Atlantis? T3-C18 (150 mm * 2.1 mm i.d., 3 μm)當作分離管柱,以0.1 % H3PO4水溶液:乙?(CH3CN)=55 %:45 %當作動相,在0.2 mL/min的流速下使用等位沖提(Isocratic Elution)進行分離。為了使樣品可達到最佳的萃取效果,本研究中也嘗試在固相萃取法的條件上進行探討,如pH值、管柱材質、沖提液等,而最佳的萃取條件為在pH2-3,使用HLB當作固相萃取管柱並利用4 %甲酸於甲醇溶液中進行沖提。本研究中羅丹明B和羅丹明6G的定量極限分別為0.5 ng/L和2 ng/L,其環境水樣中的萃取回收率在55%至90 %之間,相對標準偏差在5.5 %至17 %之間,待測物於環境水樣中的含量則在0.7 ng/L至81 ng/L之間。 Rhodamine B (RB) and rhodamine 6G (R6G) belong to the xanthene dyes, which are widely used as color additive in cosmetic, food, pharmaceutical and also used as colorant in textiles, plastic industry, etc. Although there are widely used in variety domain, many research pointed out that these compound would cause irritation to the skin, eyes and respiratory tract, moreover, the carcinogenicity, reproductive and developmental toxicity of xanthene dyes have also been reported.   A simple method was developed to detect two xanthene-type dyes, rhodamine B (RB) and rhodamine 6G (R6G) in Taiwan’s environmental water samples by high performance liquid chromatography coupled with fluorescence detector (HPLC-FLD). The contents of these two xanthene-type dyes in environmental water sample were extracted by solid-phase extraction (SPE). The optimization separation condition was Atlantis? T3-C18 (150 mm * 2.1 mm i.d., 3 μm) as separation column, and 0.1% H3PO4 solution : CH3CN=55% : 45% as mobile phase, flow rate is 0.2 mL/min for isocratic elution. The SPE condition such as various pH, SPE cartridges, and eluents were also evaluated, the optimization extraction condition was that using HLB cartridge, elute with 4 % HCOOH in MeOH at pH2-3. Experimental results indicate that the proposed method is precise and sensitive in analyzing rhodamine B and rhodamine 6G, and the limits of quantitation were 0.5 ng/L for R6G and 2 ng/L for RB. The recovery of analytes in spiked environmental water samples ranged from 55 % to 90 %, and the precision (RSD) ranged from 5.5 % to 17 %. The concentration of analytes were detected in environmental water samples ranging from 0.7 ng/L to 81 ng/L.
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