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|Title: ||新型態中孔洞材料之合成與應用：磷酸化蛋白質純化與去氧核醣核酸藥物載體之設計;Synthesis and application of a new type mesoporous silica: phosphopeptide purification and DNA drug carrier|
|Issue Date: ||2014-10-15 14:39:11 (UTC+8)|
;The first part of my research is about annular rings with controllable sizes are successfully created on the surface of COOH-functionalized mesoporous silica (namely, XC-CAR-10, where X represents the number of carbons in the diamines used) for the adsorption of metal ions. Particularly, excellent adsorption ability of 3C-CAR-10 towards Er3+ (ca. 174 μg/mg) was achieved.
Reversible phosphorylation of proteins is a common theme in the regulation of important cellular functions such as growth, metabolism, and differentiation. Although, the number of cellular phosphoproteins is relatively high, the phosphorylated residues themselves are generally of low abundance due to the sub-stoichiometric nature. Additionally, phosphopeptides with phosphate groups are able to form stable complexes with the lanthanide ions by the formation of precipitation. In this study, thus, we reported an annular mesoporous adapted with trivalent lanthanide ions Er3+ on for the applications of phosphopeptide enrichment and cleaning-up.
The second part of my research is about a new style drug carrier with designed DNA as a cap to control drug release. We synthesized mesoporous silicas with an amine group on the surface, named AM-SBA15. Due to owning positive charge of AM-SBA15, it can be formation of positive-negative interaction between AM-SBA15 and DNA phosphate groups at neutral condition. Thus, we designed a short length DNA with 17 EcoRV cutting cites as caps on the surface of AM-SBA15 mesoporous silica. According to investigation results, DNA shows a good capability for covering pores on the surface of AM-SBA15 so as to not allow fluorescein released. Remarkably, the fluorescein will be released upon EcoRV added because restriction enzyme of EcoRV digests DNA into smaller fragments. Finally, we develop a novel platform as a drug carrier and release system. Drugs will be released when composites are triggered by specific restriction enzyme.
|Appears in Collections:||[化學研究所] 博碩士論文|
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