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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/65066

    Title: 探討以活性碳進行萃取式發酵來提升Clostridium acetobutylicum 產丁醇之研究
    Authors: 王凱平;Wang,Kai-ping
    Contributors: 化學工程與材料工程學系
    Keywords: 活性碳吸附;丁醇;activate carbon;butanol;Clostridium
    Date: 2014-08-26
    Issue Date: 2014-10-15 14:39:40 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 本研究是利用活性碳對Clostridium acetobutylicum進行萃取式發酵,在發酵期間添加活性碳,降低發酵液中丁醇的濃度,藉由此種方式來改善丁醇抑制效應的問題。本次實驗在菌體生長的穩定期添加不同克數的活性碳移除丁醇,使溶液中丁醇的濃度降低,提升丁醇的最大累積濃度。
    除了添加活性碳,降低丁醇濃度對於ABE發酵的影響之外,活性碳改質也是探討的重點,利用NaOH使表面增加OH基以及在高溫400OC度的條件下,分別通入5%、10%及20%的氧氣增加活性碳表面的鹼性官能基OH來改善活性碳移除丁醇的效果,減少活性碳的使用量。;Activated carbon was used in acetone-butanol-ethanol (ABE) fermentation by Clostridium saccharoperbutylacetonicum .Improve the growth inhibition of butanol concentration(10 g/L) in ABE fermentation by adding activated carbon at the different Clostridium growth phases. When Clostridium grow into decline phase, where butanol concentration reach the maximum point, considering the addition of activated carbon time proffer the optimal timing to remove the butanol and provide a suitable growing condition. On the one hand, modification of activated carbon surface was taken by oxygen gasification with the ratio of oxygen to hydrogen(1:5、1:9、1:17) and the introduction of surface functional groups depended upon the severity of the treatment: carbonylic and phenolic type groups were introduced in all partially gasified activated carbon surface. Gasified samples were efficient to absorb butanol in ferment procedure ,and the accumulation of butanol concentration could reach to 11.52 g/L. On the other hand, NaOH treat activated carbon Surface increasing the hydroxyl and carboxyl categories for a further increase of butanol intake with the result that activated carbon could remove butanol concentration to 6.13g/L
    Appears in Collections:[化學工程與材料工程研究所] 博碩士論文

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