;Abstract The study is to develop a fermentation strategies by liquid monocultural two strains of fungi A.niger and T.reesei production hydrolysis enzymes, and then to increase the inoculum way to enhance the production of enzymes, and to replace the nitrogen compound source by self-production of bean dregs to reduce the cost of the fermentation process, when entering the saccharification stage,after study the physical conditions and the timing of saccharification,and then by Mixing a way to mix the enzyme fermentation broth of two fungi called AT co-saccharification. To achieve maximum effect of hydrolysis of AT enzyme solution,at co-saccharification stage the rice straw was added twice the saccharification stage of the hydrolyzate,used in the final stage of ethanol fermentation by Saccharomyces cerevisiae.Experimental results show that, AT mixed enzyme solution of the present study the maximum cellulase activity was found to be 0.511 FPU / mL, at optimum condition and from the saccharification of 3g rice straw in the second stage were obtained the maximum reducing sugar accumulation of 21.25g / L, the hydrolysis efficiency of 85.2 %, the amount of ethanol produced of 7.172 g / L in the final stage, the conversion yield was 76.7%.
