水稻CCR4基因的功能分析- 繁衍大量表現和靜默表現的基因轉殖水稻;Functional analysis of CCR4 genes in rice -generation of overexpression and RNAi knock down transgenic rice plant

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Title:	水稻CCR4基因的功能分析- 繁衍大量表現和靜默表現的基因轉殖水稻;Functional analysis of CCR4 genes in rice -generation of overexpression and RNAi knock down transgenic rice plant
Authors:	何浩恩;Haw-En Ho
Contributors:	生命科學研究所
Keywords:	水稻;基因轉殖;降解;降解腺呤;deadenylation;decay;turnover;mRNA;transgenic rice
Date:	2009-01-12
Issue Date:	2009-09-22 10:20:57 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	基因表現的調控可以是在轉錄階層或是後轉錄修飾階層，甚至兩者都可影響。在真核中mRNA主要的降解機制是由尾端poly (A)的降解開始，此步驟是各樣mRNA降解的速率決定步驟，並在各樣的生物種間都可以發現這樣的情形。在酵母菌中， CCR4-NOT (carbohydrate catabolism repression 4-not)的複合體中的CCR4蛋白質，被認為扮演著降解 mRNA deadenylase的關鍵角色。然而，在植物中CCR4的功能還是非常不清楚。因此本實驗利用單子葉植物，水稻，來分析植物中，CCR4對mRNA 降解的影響。本實驗利用大量表現OsCCR4-1和 OsCCR4-2和RNAi knock down的轉殖水稻，來分析植物中CCR4對mRNA降解的影響。我們成功得到T1代的靜默植株和T2代的大量表現轉殖植株。分析癒傷組織 (callus)的OsCCR4-1和 OsCCR4-2表現量，初步結果認為沒有參予在糖調控基因α-amylase表現的pathway上。進一步，之後可找到T2代轉殖植株和T3 homologous 的植物，將來可以分析OsCCR4在水稻中的生化功能。 In general, gene expression is regulated at either a transcriptional level or a post-transcriptional level, or even both. In eukaryotes, the major mechanism of mRNA degradation involves a poly A tail deadenylation. The deadenylation is the rate-limiting step in many mRNA degradation events in a wide-range of organisms. In yeast, the CCR4 (carbohydrate catabolism repression 4) protein, a part of the CCR4-NOT complex, have been proposed that play a prominent role as deadenylase in mRNA degradation. However, the function of CCR4 in plants is not clear yet. In this study we use the monocot model plant, rice, to further investigate the general role of plant CCR4 in mRNA degradation. The OsCCR4-1 and OsCCR4-2 overexpression and RNAi knock down transgenic rice were generated, and they will be analyzed for the functions of OsCCR4-1 and OsCCR4-2 in rice subsequently. The T1 RNAi plant and T2 overexpression rice plant have been identified. Continuously we will identified the T2 transgenic plant and T3 homologous transgenic rice plant.The function of OsCCR4-1 and OsCCR4-2.will be analyzed in the future.
Appears in Collections:	[Graduate Institute of Life Science] Electronic Thesis & Dissertation

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