本實驗旨在使用mouse lewis lung carcinoma(LL/2)癌症細胞與chlorin e6 (Ce6)光敏化藥物製備癌症疫苗，研究改變不同Ce6劑量與光動力療法處理後的等待時間對於癌症細胞表現熱休克蛋白70的改變，以最佳化疫苗的效果。並藉由收集外排體誘導巨噬細胞釋放TNF-α以驗證疫苗刺激免疫反應的效果。;Over past decade, Photodynamic therapy(PDT) is one of most potential way in cancer research. In classic PDT, photosensitizer(PS) is injected into tumor region, then expose to speci c wavelength of light to excite PS to induce singlet oxygen dealing damage directly on tumor cell. Recent study shows that treating PDT on surgical resection tumor or culture tumor cell ex-vivo make cancer vaccine which stimulate immune system to cure and prevent caner.
The mechanism of PDT-treated vaccine is not clear, but research shows that the level of heat shock protein 70(hsp70) expression in cancer cell after PDT treated is correlate with their immunogenicity. In addition, after PDT treated, cancer cell release exosome, a kind of membrane-bound nanovesicle, induce macrophage to release TNF-α as an indicator of macrophage activation.
In this research, we choose mouse lewis lung carcinoma and chlorin e6 to develop cancer vaccine. We change concentration of Ce6 and recovering time after PDT to optimize vaccine by detecting cell surface hsp70, and induce macrophage release TNF-α by exosome to test immunogenicity of vaccine.