English  |  正體中文  |  简体中文  |  Items with full text/Total items : 73032/73032 (100%)
Visitors : 23189355      Online Users : 543
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/65302

    Title: 藥物劑量與復原時間影響光動力療法疫苗之功效的系統性研究;Systematic Study of the Dependence of PDT Vaccine Efficacy on PDT Dosage and Recovering Time
    Authors: 劉忠翰;Liu,Jhong-Han
    Contributors: 物理學系
    Keywords: 光動力療法;癌症疫苗;外排體;免疫原性細胞凋亡;Photodynamic therapy;cancer vaccine;exosome;immunogenicity apoptosis
    Date: 2014-08-29
    Issue Date: 2014-10-15 14:49:28 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 光動力療法是近十年來癌症治療研究中一個很有潛力的領域,傳
    波段的光,激發光敏化藥物產生單態氧(singlet oxygen)以直接破壞腫瘤細胞。近期研究發現,體外培養癌症細胞或手術將活體內的腫瘤


    本實驗旨在使用mouse lewis lung carcinoma(LL/2)癌症細胞與chlorin e6 (Ce6)光敏化藥物製備癌症疫苗,研究改變不同Ce6劑量與光動力療法處理後的等待時間對於癌症細胞表現熱休克蛋白70的改變,以最佳化疫苗的效果。並藉由收集外排體誘導巨噬細胞釋放TNF-α以驗證疫苗刺激免疫反應的效果。;Over past decade, Photodynamic therapy(PDT) is one of most potential way in cancer research. In classic PDT, photosensitizer(PS) is injected into tumor region, then expose to speci c wavelength of light to excite PS to induce singlet oxygen dealing damage directly on tumor cell. Recent study shows that treating PDT on surgical resection tumor or culture tumor cell ex-vivo make cancer vaccine which stimulate immune system to cure and prevent caner.

    The mechanism of PDT-treated vaccine is not clear, but research shows that the level of heat shock protein 70(hsp70) expression in cancer cell after PDT treated is correlate with their immunogenicity. In addition, after PDT treated, cancer cell release exosome, a kind of membrane-bound nanovesicle, induce macrophage to release TNF-α as an indicator of macrophage activation.

    In this research, we choose mouse lewis lung carcinoma and chlorin e6 to develop cancer vaccine. We change concentration of Ce6 and recovering time after PDT to optimize vaccine by detecting cell surface hsp70, and induce macrophage release TNF-α by exosome to test immunogenicity of vaccine.
    Appears in Collections:[物理研究所] 博碩士論文

    Files in This Item:

    File Description SizeFormat

    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback  - 隱私權政策聲明