藥物劑量與復原時間影響光動力療法疫苗之功效的系統性研究;Systematic Study of the Dependence of PDT Vaccine Efficacy on PDT Dosage and Recovering Time

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題名:	藥物劑量與復原時間影響光動力療法疫苗之功效的系統性研究;Systematic Study of the Dependence of PDT Vaccine Efficacy on PDT Dosage and Recovering Time
作者:	劉忠翰;Liu,Jhong-Han
貢獻者:	物理學系
關鍵詞:	光動力療法;癌症疫苗;外排體;免疫原性細胞凋亡;Photodynamic therapy;cancer vaccine;exosome;immunogenicity apoptosis
日期:	2014-08-29
上傳時間:	2014-10-15 14:49:28 (UTC+8)
出版者:	國立中央大學
摘要:	光動力療法是近十年來癌症治療研究中一個很有潛力的領域，傳統的光動力療法為:先將光敏化藥物注射至體內，在腫瘤區域照射對應波段的光，激發光敏化藥物產生單態氧（singlet oxygen）以直接破壞腫瘤細胞。近期研究發現，體外培養癌症細胞或手術將活體內的腫瘤細胞直接取出，在體外進行加藥及照光的步驟，可製作出癌症疫苗，將此疫苗注射入體內會刺激免疫反應，進而達到治療和預防癌症的效果。目前對於此疫苗激活免疫系統的機制尚未完全明朗，但研究指出受刺激的細胞釋放熱休克蛋白70的量與疫苗效果呈正相關，另外，細胞受到光動力療法刺激後會產生帶有熱休克蛋白70與癌細胞膜表面抗體的外排體(exosome)，此外排體上的熱休克蛋白70會吸引巨噬細胞吞噬外排體並釋放TNF-α。本實驗旨在使用mouse lewis lung carcinoma(LL/2)癌症細胞與chlorin e6 (Ce6)光敏化藥物製備癌症疫苗，研究改變不同Ce6劑量與光動力療法處理後的等待時間對於癌症細胞表現熱休克蛋白70的改變，以最佳化疫苗的效果。並藉由收集外排體誘導巨噬細胞釋放TNF-α以驗證疫苗刺激免疫反應的效果。;Over past decade, Photodynamic therapy(PDT) is one of most potential way in cancer research. In classic PDT, photosensitizer(PS) is injected into tumor region, then expose to speci c wavelength of light to excite PS to induce singlet oxygen dealing damage directly on tumor cell. Recent study shows that treating PDT on surgical resection tumor or culture tumor cell ex-vivo make cancer vaccine which stimulate immune system to cure and prevent caner. The mechanism of PDT-treated vaccine is not clear, but research shows that the level of heat shock protein 70(hsp70) expression in cancer cell after PDT treated is correlate with their immunogenicity. In addition, after PDT treated, cancer cell release exosome, a kind of membrane-bound nanovesicle, induce macrophage to release TNF-α as an indicator of macrophage activation. In this research, we choose mouse lewis lung carcinoma and chlorin e6 to develop cancer vaccine. We change concentration of Ce6 and recovering time after PDT to optimize vaccine by detecting cell surface hsp70, and induce macrophage release TNF-α by exosome to test immunogenicity of vaccine.
顯示於類別:	[物理研究所] 博碩士論文

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