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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/66201


    Title: 利用班馬魚胚胎模式探討得克利、菲克利、三泰芬三種農藥干擾性腺荷爾蒙表現量之可能性;Studying the Effects of Tebuconazole,Hexaconazole,Triadimefon on the Expression of Gene-Related to Sexual Hormones in Zebrafish Embryo
    Authors: 陳師慶
    Contributors: 國立中央大學生命科學系
    Keywords: 漁業;生物技術(農);zebra fish embryo;Hormone-related genes;TEBUCONAZOLE;HEXACONAZOLE;TRIADIMEFON
    Date: 2015-01-08
    Issue Date: 2015-01-13 11:11:38 (UTC+8)
    Publisher: 行政院農業委員會
    Abstract: 研究期間:10301~10312;Assessment of endocrine disruption currently relies on testing strategies involving adult vertebrates. In order to minimize the use of animal tests according to the 3Rs principle of replacement, reduction and refinement, we propose a transcriptomics and fish embryo based approach as an alternative to identify and analyze an estrogenic activity of environmental chemicals. For this purpose, the suitability of different time postfertilization zebrafish  embryos to test for estrogenic disruption was evaluated. The embryos were exposed to the pesticide and subsequently analyzed by  quantitative real-time PCR.  Overall, the study demonstrated that combining fish embryo testing with qRT-PCR can deliver abundant information about the mechanistic effects of endocrine disrupting chemicals, rendering this strategy a promising alternative approach to test for endocrine disruption in a whole organism in-vitro scale system
    Relation: 財團法人國家實驗研究院科技政策研究與資訊中心
    Appears in Collections:[生命科學系] 研究計畫

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