生物啟發兩性雙離子自組裝抗汙塗層;Bio-inspired Zwitterionic Assemblies for Robust Antifouling Coatings

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題名:	生物啟發兩性雙離子自組裝抗汙塗層;Bio-inspired Zwitterionic Assemblies for Robust Antifouling Coatings
作者:	朱思澔;Chu,Sz-Hau
貢獻者:	生物醫學工程研究所
關鍵詞:	非特異性吸附;自組裝薄膜;兩性雙離子氨基酸;光趨動氧化奈米材料;光熱治療;non-specific adsorption;self-assembled monolayers;zwitterionic amino acid;nanomaterials colloidal stability;photothermal therapy;photo induced oxidation
日期:	2015-07-29
上傳時間:	2015-09-23 10:54:57 (UTC+8)
出版者:	國立中央大學
摘要:	植入性醫療器材，由於長時間與血液、體液接觸，容易因為表面的蛋白質、微生物的非特異性吸附，進而引發儀器被組織包覆，效能、靈敏性受損，甚至造成凝血反應、微生物感染、血栓形成等問題。而目前的解決此問題的其中一項辦法為在醫療器材表面修飾一層抗貼附塗層以降低非特異性吸附，避免併發症的發生。其中以自組裝 (Self-Assembling)的修飾方法由於其步驟簡單、處理快速等特性尤為受到矚目。然而，也有許多學者認為，自組裝膜的表面穩定性與抗汙功效，會是其使用上的一大問題。在本研究中，我們利用天然兩性雙離子氨基酸─半胱胺酸(Cysteine)、自行合成之半胱胺酸四級銨衍生物─半胱胺酸甜菜鹼(Cysteine betaine)和市面上常使用之親水性抗貼附材料─聚乙二醇(Polyethylene Glycol)，自組裝於金基板上，利用水接觸角、X射線光電子能譜、掃描式隧道顯微鏡觀察修飾後表面的親水性質、表面元素組成及分子排列情形。並藉由細胞毒性試驗MTT、革蘭氏陽性菌表皮葡萄球菌（Staphylococcus epidermidis）和陰性菌綠膿桿菌（Pseudomonas aeruginosa）細菌貼附、3T3纖維母細胞貼附和蛋白質貼附，比較其化學穩定度、應用範圍及抗貼附能力。最後，將其修飾在空心金銀奈米殼(Hollow Ag@Au nanoshells) 上，調控溫度、鹽濃度、離子濃度、蛋白質貼附等變因，觀察其膠體穩定性並應用於近紅外光光熱治療。最後開發出一極具潛力之生物衍生兩性雙離子自組裝抗汙塗層。;Implantable medical devices are widely used in clinical medicine. However, they directly contact to blood or body fluid and nonspecific adsorption of protein or microorganisms may occur. This process will lead to decreased efficiency or sensitivity which further facilitates coagulation, infection or thrombus formation. Modification the surface of medical device with antifouling coatings may decrease the possibility of nonspecific adsorption and prevent formation of complications. Among them, modification by self-assembling is an easy and efficient way to improve the biocompatibility. Nevertheless, the stability and effectiveness of self-assembled monolayers (SAMs) is also an issue for long-term biomedical applications. In this study, we used cysteine (natural sulfur-containing zwitterionic compounds), cysteine betaine (derivative of cycteine with quaternary ammonium group at its terminal end), and polyethylene glycol (a widely used hydrophilic antifouling material) to decorate with Au substrate through Au-thiol interaction. We arranged contact angle goniometer, X-ray photoelectron spectroscopy (XPS), and scanning tunneling microscope (STM) to examine the hydrophilic property, surface elemental composition and molecular arrangement situation in this study. MTT cytotoxicity assays, bacterial adsorption test, 3T3 fibroblast cel�#(~sorption, and protein adsorption were also arranged to compare the range of application and antifouling capacity. Finally, the modification was applied to hollow gold silver nanoshells. After regulating temperature, ion concentration, and protein adsorption, we tested the colloid stability. This technique then applied to near-infrared photoelectron thermal therapy and developed into bio-derived zwitterionic assemblies for antifouling coatings with high potential in clinical implications.
顯示於類別:	[生物醫學工程研究所 ] 博碩士論文

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