本研究為利用疏水性海綿對Clostridium acetobutylicum進行萃取式發酵，並探討其影響及可行性。Clostridium acetobutylicum在進行ABE發酵時，若發酵液中丁醇抑制物濃度達到10 g/L就會對菌體產生嚴重的抑制影響而失活，因此藉由疏水性海綿透過萃取式發酵以降低丁醇帶來的抑制效應，並提升丁醇產量。 本實驗將探討一項新的製程，利用疏水性PDMS海綿作為抑制物萃取劑，在菌體發酵至72小時進行丁醇萃取，海綿萃取劑對發酵液重量百分比為3.5%，萃取時間為1.5小時。經過萃取後，丁醇濃度由6.23 g/L下降至5.03g/L，且在84小時達最高產量10.72 g/L。與控制組相比，丁醇總產量提升15.02%，丁醇濃度達最大值之發酵時間由96小時提前至84小時，雖然菌體在丁醇萃取後沒有增加的趨勢，但可減緩其失活速度，因此利用PDMS疏水海綿作為萃取劑之製程可行性，值得深入探討。 ;In this research, we use hydrophobic Polydimethylsiloxane(PDMS) sponge to extract butanol from fermentation of Clostridium acetobutylicum, then discuss its effect and feasibility. When the concentration of butanol get 10 g/L in ABE fermentation, the Clostridium acetobutylicum will be inhibited by the product, butanol, and lose activation. The extractive fermentation will improve this problem by reducing the concentration of butanol. The experiment will explore a new process using PDMS sponge as the extractant. we extract by PDMS sponge (3.5wt%)in 1.5 hours when the fermentation gets to 72 hours, the concentration of butanol reduced from 6.23 g/L to 5.03 g/L. After 84 hours, the maximum yield of butanol is 10.72 g/L and higher than controlled experiment (9.32 g/L), and time of the butanol reach to maximum concentration is advanced from 96 to 84 hours compared to control experiment. The biomass of bacterium is no significant change after extracting, but the deactivation of bacterium is slow down. Therefore, the process feasibility of using PDMS hydrophobic sponge as extractant, is worth in-depth study.
