本研究旨在探討細胞固定化於厭氧氨氧化程序的應用與影響。過程中首先以污水處理廠之活性污泥為植種源，馴養並富集所需之厭氧氨氧化菌；之後以批次試驗探討厭氧氨氧菌於懸浮和包埋培養下除氮活性之差異與可能原因；最後評估以固定化技術結合部份硝化與厭氧氨氧化程序於單一反應槽進行完全自營性除氮之可行性。實驗結果顯示以活性污泥為植種源，在提供適合厭氧氨氧化菌生長的環境下，可成功地自活性污泥增殖培養出厭氧氨氧化菌。批次試驗的比較結果顯示在低氮濃度負荷下(50 mg-N/L)，雖然懸浮系統和褐藻膠包埋系統在總氮去除率上有所不同(74±2.29% vs.100±13.86%)，但是當把系統內的細胞濃度考慮進去時，則兩系統的去除速率則幾乎相同(0.0148±0.0004 mg-N/mg-VSS/day vs. 0.0121±0.0017 mg-N/mg-VSS/day)，說明在先前研究所觀察到的差異很可能是由不同的細胞密度所導致；不過，當包埋系統於高氮濃度負荷下試驗時(250 mg-N/L)，原先具活性的ANAMMOX 則受到抑制，推測是由於基質在褐藻膠的介質環境中傳輸時受到質傳限制所造成。而於雙膜單槽的研究結果證實，在不外添加亞硝酸鹽，以雙薄膜系統結合部分硝化和厭氧氨氧化程序處理高科技產業廢水確實可行，且系統在有限的溶氧條件下，可藉由曝氣強度控制總氮之去除。;This study was aimed to investigate the effect and potential applicability of cell immobilization on nitrogen removal predominantly modulated by the anaerobic ammonium oxidation (ANAMMOX) process. Specifically, the study included (i) establishment of the ANAMMOX enrichment cultures using activated sludge as a seeding source, followed by (ii) comparison of the nitrogen removal efficacies between suspended and alginate-entrapped enrichments on a per-cell basis under batch-growth conditions, as well as (iii) evatulation of the feasiability of using the immobilization technique for an integrated partial-nitrification and ANAMMOX process in a single tank for the treatment of high-tech wastewater. Results show that while the color of the enrichment did not appear to be reddish, multiple lines of evidence derived from analyses of water quality and molecular characterization revealed that the ANAMMOX cultures were successfully enriched from activated sludge. Further, although the total nitrogen removal efficacy of the system as a whole was different between suspended and embedded cultures under batch-growth, low nitrogen-loading conditions, it was nearly identical on a per-cell basis for both systems, suggesting that the differences observed previously might have been resulted primarily from differential cell densities. Surprisingly, while the cells remained viable, increases in nitrogen-loadings resulted in inhibition of ANAMMOX activity in the embedded cultures, presumably due to the mass transfer limit encountered in alginate matrix. Lastly, it was demonstrated that using a dual-membrane system integrating partial nitrification and ANAMMOX processes without a direct supplement of nitrite to treat high-tech wastewater was feasible, and the extent to which the nitrogen removal seemed to be controlled by aeration intensity.
