本文研製一新型光學生物感測器：導模共振(guided mode resonance, GMR)光學生物感測器。本元件利用半導體技術，製作一次波長光柵結構，並利用其產生的窄帶高反射率之效果，及其光柵表面區域之高敏感度，應用於共種生化反應，本元件具有以下優點：免標定、微小化、可高產量、即時偵測、易與其它半導體元件接合之優點。 本研究已將GMR元件組裝至流體系統內，形成GMR流體晶片。除了方便將整個生化實驗於晶片內進行外，所使用到的檢體量也相對的減少，並發展出一套即時檢測平台，縮短了檢測所需時間。目前於晶片本身已能檢測出折射率變化量小於0.001，且偵側去氧核醣核酸之雜交行為，除了可即時觀察出有無雜交反應外，其有無雜交差異量更達9倍之多。對於胜肽合成，能成功偵測出每段長度約0.3nm之胺基酸接合反應及其反應之分子動力學行為。We report a novel method for detecting biomolecular interaction which utilizes the guided-mode resonance (GMR) phenomenon with narrow-band high reflection efficiency characteristics. The structure of GMR device generally consists of two stages — upper grating layer and waveguide layer. GMR device and flow cell are combined to from a GMR flow cell system. The system has several advantages such as label-free, minimizing, real time monitoring, high throughput, low cost, and the potential to become portable. We show the system experimentally with the ability to detect the refractive index variations <0.001,the amino acid thickness variations <0.3nm. It also can detect DNA hybridization and measure molecular interaction.
