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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/69420

    Title: 葉綠素雙光子螢光超光譜影像於光合作用研究之應用;Chlorophyll Two-photon Fluorescence Hyperspectral Imaging Applied to Photosynthesis Research
    Authors: 蘇柏宇;Su,Poyu
    Contributors: 光電科學與工程學系
    Keywords: 雙光子螢光;超光譜;葉綠素螢光;Two-photon fluorescence;Hyperspectral;Chlorophyll fluorescence
    Date: 2016-01-28
    Issue Date: 2016-03-17 20:35:56 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 葉綠素螢光已被認為可有效作為高等植物研究應用,作為非侵入性的方法評斷植物生理狀況,本研究使用線掃描式雙光子螢光超光譜顯微系統取得葉片樣本的葉綠素螢光超光譜影像,超光譜影像具有一維光譜與三維空間資訊,可取得最厚樣本深度為葉片樣本表面往下120 μm。
    ;Chlorophyll fluorescence is considered as a non-invasive method to monitor the physiological state of plants and is capable to apply for higher plants researches. A home-built line scanning two-photon fluorescence hyperspectral microscopy is used to record the chlorophyll fluorescence hyperspectral images containing 3D spatial and 1D spectral information in this research. The hyperspectral images within 120 μm beneath the leaf surface can be recorded.
    In this research, the principle component analysis method and cryogenic fluorescence spectrum measurement are applied to analyze the spectrum bases of Photosystem I and Photosystem II. To correct the re-absorption effect in spectrum analysis, absorption spectrum of intact leaf is measured and used to build the fitting function. Using least squares method, the fluorescence spectra of mesophyll cells at various locations in three-dimensional are fitted and analyzed.
    Through the errors between the measured and fitted fluorescence spectra, the fitting method used in this research is verified to be applicable for analysis of chlorophyll fluorescence.
    Appears in Collections:[光電科學研究所] 博碩士論文

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