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|Title: ||掃描式二倍頻結構照明顯微術;Second Harmonic Generation Scanning Structured Illumination Microscopy|
|Issue Date: ||2016-06-04 13:00:29 (UTC+8)|
二倍頻訊號是同調訊號，成像理論與螢光的成像理論不同，本論文基於同調訊號的成像理論建立了掃描式二倍頻結構照明顯微術的成像原理，並證實了在重建後的影像中具有較高頻的資訊，透過模擬，在1047 nm、873 nm、748.6 nm和655 nm這四種不同週期的條紋下提升的解析度分別為1.28、1.35、1.44、1.52倍；在實驗上則利用雞翅的肌腱組織成功的在1047 nm、873 nm條紋週期下於X方向取得1.29和1.38倍的解析度提升，Y方向則取得1.3和1.4倍的解析度提升。;Two-photon microscopy has an outstanding optical sectioning capability due to its nonlinear excitation process. While observing tendon tissues with its second harmonic generation microscopy, because of the non-centrosymmetric structure, tendons can be observed directly. In addition, the excited process has no energy level transitions, meeting the conservation of momentum, therefore the sample will not be damaged by the light source, making it well suited for long time observations.
Unlike fluorescents, second harmonic signals are under the conditions of no energy level transitions, thus it does not apply to the fluorescent signal-based super-resolution microscopies, however, it could be combined with structured illumination microscopy. The original structured illumination microscopy was based on a wide field setup, yet two-photon microscopy systems require extremely high excitation intensities to produce the two-photon excitation on its samples, consequently, scanning systems were chosen to overcome such issue. This paper will combine the scanning second harmonic generation microscope and structured illumination microscopy to improve the resolution.
Second harmonic signals are coherent signals, its imaging theory is different to the incoherent signal’s. In this paper, it is proven that the reconstruction image has a higher frequency information based on the Second Harmonic Generation Scanning Structured Illumination Microscopy theory. In simulation, under four different periods: 1047 nm, 873 nm, 748.6 nm and 655 nm, the resolution is enhanced by 1.28, 1.35, 1.44, and 1.52 times respectively; in experiments, by using chicken wings tendon as samples, resolution were improved by 1.29 and 1.38 times; and in the Y direction, 1.3 and 1.4 times the resolution improvement were achieved.
|Appears in Collections:||[光電科學研究所] 博碩士論文|
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