生物感測器目前在醫學檢測上已經有極大的幫助。其中葡萄糖感測器已成為方便且成熟之檢測用品，全世界每年之消耗量極為龐大，使此產品極具有經濟價值。本研究中為了製備低成本之金電極葡萄糖感測器，先使用含鈀觸媒之油墨利用網印方式作為基板，探討其硬度及鈀觸媒含量的影響。再來製作特別的反應槽，採用化學電鍍的方式將鎳、鈀及金三種金屬分別鍍在基板之上，最終目的使其增加產品對血液之導電性。化學電鍍方面，比較溫度、時間、金屬硬度及厚度對產品穩定性之影響。之後在金金屬表面點上酵素，比較使用金電極與其他市售碳電極葡萄糖感測器等之差異以及準確度。最後將之放大量化作業，使其更加擁有商業價值。研究中以聚焦離子束與電子束顯微鏡(FIB)、掃描式電子顯微鏡(SEM)和化學分析電子儀(XPS)等儀器鑑定產品之物理、化學特性和表面性質。結果顯示：鎳鈀金三個反應槽在特定的溫度與時間區間中會有較好的化學鍍品質，最終測試的結果發現鎳的厚度在極薄的情況下整體的準確度會上升達到工業需求的標準。並且金電極所做出之葡萄糖感測器，測出之準確性較其他電極良好，可積極用於市面上。;With the rapid development of technology, biosensors have been used extensively in medical detecting. Glucose biosensors, which have become the convenient and mature product, have great economic value because of huge consume in the world. In this study, in order to prepare lower cost gold electrode glucose biosensors, the screen printed substrate which contained palladium catalyst was prepared. The effects of contents of palladium on the performance of biosensor was investigated. A reactor for electroless plating was designed. Nickel, palladium and gold were deposited on the substrate in order to increase the conductivity of electron passed through the sample. The operating parameters such as temperature, time, hardness and thickness were changed to study their effects on the stability of product. The samples were then doped by the enzyme to check the performance of glucose biosensors. The samples were characterized by focused ion beam (FIB), scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). The results illustrated that the better operating conditions of electroless reactors for nickel, palladium and gold was at the region of temperature and dipping time.