近年來有各種生物標記物( biomarkers )的檢測方法被發展與推廣。這些檢測方法包括利用光學、電學、質量等運作原理的生物感測器( biosensors )來偵測DNA、RNA、蛋白質等所謂之生物標記分子。在眾多的生物感測器中，因為矽奈米線場效電晶體相較於其他類別的生物感測器有著超靈敏、具選擇性、即時且免螢光標定的優勢，因此被廣泛的研究與發展，也使得矽奈米線場效電晶體在未來醫學診斷方面有很大的應用潛力。現今矽奈米線場效電晶體的生物分子固定化流程大多採用整片式固定法( all area modification )，然而以這種方式固定上生物受體只能檢測一種生物標記物。 為了解決這個問題，我們開發了「多點固定化系統」。此系統可以將多種生物受體( bioreceptors )溶液點在晶片上的每個元件上，若矽奈米線場效電晶體有更多種生物探針固定於其上，我們就能在同一個晶片上檢測多種生物標記物就是所謂的高通量檢測。相較於整片式固定法，節省成本與實驗的時間是一個很大的優勢。 在本研究，我們首先探討DNA在不同鹽離子濃度下對雜交的影響，接下來我們再探索探針溶液中的甘油比例與探針濃度於多點固定化系統的實驗最佳條件，最後我們嘗試在同一片晶片上固定不同種類的生物探針來進行核酸檢測。 ;In recent years, various biomarker detection methods have been invented and studied. Optical, mass, electrical and etc… biosensors are common for monitoring DNA, RNA, protein, and so on. Among them, Silicon nanowire field effect transistor (SiNW-FET) is the one that has been extensively studied and developed due to its distinguished advantages over the other biosensors. SiNW-FET is ultrasensitive, selective, real-time and label-free detection platform. Because of these features, SiNW-FET has a great potential in the future of clinical diagnostics. In the current process of SiNW-FET immobilization, we mostly use all area modification. However, if we use this method to immobilize bioreceptors on SiNW-FET, the device can detect only one kind of biomarker. To solve this problem, we develop a technique called “multi-point immobilization system”. Multi-point immobilization system can overcome the above-described problems because this system can spot many kinds of bioreceptor solution on each device. If there are more bioreceptor probes on SiNW-FET, we can detect more categories of biomarker targets on same device. Saving costs and experimental time are comparative advantage with all area modification. In this research, first we investigate the effects of salt ion concentration on hybridizing. Then we explore the best ratio of glycerol in probe solution and optimal probe concentration in multi-point immobilization system. Finally we try to immobilize different probe on same device to measure electrical signal.
