English  |  正體中文  |  简体中文  |  Items with full text/Total items : 74010/74010 (100%)
Visitors : 24678382      Online Users : 249
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version

    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/71299

    Title: 矽奈米線場效電晶體多點之核酸檢測研究;Development of multi-point immobilization system on silicon nanowire field effect transistor
    Authors: 曾柏偉;Tseng,Po-Wei
    Contributors: 化學工程與材料工程學系
    Keywords: 場效電晶體;核酸分子;奈米線;微陣列;field effect transistor;nucleic acids;nanowire;microarray
    Date: 2016-07-22
    Issue Date: 2016-10-13 12:41:21 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 近年來有各種生物標記物( biomarkers )的檢測方法被發展與推廣。這些檢測方法包括利用光學、電學、質量等運作原理的生物感測器( biosensors )來偵測DNA、RNA、蛋白質等所謂之生物標記分子。在眾多的生物感測器中,因為矽奈米線場效電晶體相較於其他類別的生物感測器有著超靈敏、具選擇性、即時且免螢光標定的優勢,因此被廣泛的研究與發展,也使得矽奈米線場效電晶體在未來醫學診斷方面有很大的應用潛力。現今矽奈米線場效電晶體的生物分子固定化流程大多採用整片式固定法( all area modification ),然而以這種方式固定上生物受體只能檢測一種生物標記物。
    為了解決這個問題,我們開發了「多點固定化系統」。此系統可以將多種生物受體( bioreceptors )溶液點在晶片上的每個元件上,若矽奈米線場效電晶體有更多種生物探針固定於其上,我們就能在同一個晶片上檢測多種生物標記物就是所謂的高通量檢測。相較於整片式固定法,節省成本與實驗的時間是一個很大的優勢。
    ;In recent years, various biomarker detection methods have been invented and studied. Optical, mass, electrical and etc… biosensors are common for monitoring DNA, RNA, protein, and so on. Among them, Silicon nanowire field effect transistor (SiNW-FET) is the one that has been extensively studied and developed due to its distinguished advantages over the other biosensors. SiNW-FET is ultrasensitive, selective, real-time and label-free detection platform. Because of these features, SiNW-FET has a great potential in the future of clinical diagnostics. In the current process of SiNW-FET immobilization, we mostly use all area modification. However, if we use this method to immobilize bioreceptors on SiNW-FET, the device can detect only one kind of biomarker. To solve this problem, we develop a technique called “multi-point immobilization system”. Multi-point immobilization system can overcome the above-described problems because this system can spot many kinds of bioreceptor solution on each device. If there are more bioreceptor probes on SiNW-FET, we can detect more categories of biomarker targets on same device. Saving costs and experimental time are comparative advantage with all area modification. In this research, first we investigate the effects of salt ion concentration on hybridizing. Then we explore the best ratio of glycerol in probe solution and optimal probe concentration in multi-point immobilization system. Finally we try to immobilize different probe on same device to measure electrical signal.
    Appears in Collections:[化學工程與材料工程研究所] 博碩士論文

    Files in This Item:

    File Description SizeFormat

    All items in NCUIR are protected by copyright, with all rights reserved.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 隱私權政策聲明