本研究為利用Polydimethylsiloxane海綿對Clostridium acetobutylicum發酵液進行連續萃取發酵，並探討其效益及可行性。Clostridium acetobutylicum進行ABE發酵時，若其發酵液中丁醇濃度達到8 gL-1將使菌體進入內孢子狀態，不再生產丁醇，產生嚴重的抑制效應，進而導致失活。因此藉由將發酵液多次以蠕動幫浦引流通過裝有PDMS海綿之容器的方式，目的在於降低發酵液的丁醇濃度，並討論於適當時機下替換新鮮發酵液，以達提升總丁醇產率與產量之目的。 本研究實驗利用PDMS海綿，用以萃取抑制物，於菌體發酵達48小時後進行丁醇萃取，發酵液之工作體積為150 ml以蠕動幫浦抽出並注入裝有重量為10 g的PDMS海綿之容器中，最後回流至厭氧發酵罐，萃取時間為15分鐘。後於第60小時開始間隔24小時將50 mL發酵液抽出替換為同等體積之新鮮液態培養基。與控制組相比，丁醇總產量由1.725 g提升至7.89 g，而總產率由0.087 gL-1 hr-1略為成長至0.092 gL-1 hr-1，並使發酵時間從108小時延長至192小時，足見PDMS海綿與連續萃取發酵製程不僅可減緩其失活速度，並因海綿降低發酵液中的丁醇濃度，排除了抑制效應，而使菌體快速生長，以利提升丁醇之產量與產率，故利用PDMS海綿作為萃取物之研究，值得深入探討。 ;In this research, we extract butanol from the continuous fermentation of Clostridium acetobutylicum with Polydimethylsiloxane (PDMS) sponge , and discuss its effect and feasibility. As the concentration of butanol reach about 10 gL-1 in ABE fermentation, Clostridium acetobutylicum will be inhibited by its product, butanol, and lose its activation. It will become into a state of endospore and stop to product butanol. Thus, we will reach the target of promoting the productivity of butanol by circulating the broth into PDMS sponge system by peristaltic Pump and refresh the broth in suitable moment. PDMS sponge is used to extract the inhibitor in this research. When the fermentation reach 48 hours, it will be extracted butanol every 12 hours. The volume of working fluid of the broth is 150 mL. It will be inject into the system including 10 g PDMS sponge for 15 minutes by peristaltic Pump and reflow. It will be refresh the 50 mL broth into liquid medium in same volume. In contrast to the control, the total productivity of butanol is promoted from 1.725 to 7.89 g. The fermentation period is expanded from 108 to 192 hours. Because of PDMS sponge and the continue extractive fermentation, It will not only be slow the losing of the activation down, but also cut the inhibit effect off. And the total productivity of butanol is increased. Therefore, the process feasibility of using PDMS hydrophobic sponge as an extractant, is worth in-depth study.
