探討紅光光照及木材精油的添加對牛樟芝生產三萜類之影響;Effect of red light and addition of wood essential oil on the triterpenoids of Antrodia cinnamomea in submerged cultures

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Title:	探討紅光光照及木材精油的添加對牛樟芝生產三萜類之影響;Effect of red light and addition of wood essential oil on the triterpenoids of Antrodia cinnamomea in submerged cultures
Authors:	林雯惠;Lin,Wen-Huei
Contributors:	化學工程與材料工程學系
Keywords:	牛樟芝;木材精油;三萜類;紅光
Date:	2016-08-25
Issue Date:	2016-10-13 12:46:55 (UTC+8)
Publisher:	國立中央大學
Abstract:	牛樟芝，是一種台灣特有的藥用菇類。由於牛樟芝的藥理益處，近年來為世人所關注，其中又以抗癌、抗腫瘤的功效最受矚目。許多研究陸續指出其含有的活性成分，尤其是牛樟芝特有的二次代謝產物三萜類，經實驗證實其主要功能有抑制癌細胞的生長、抑制組織胺的釋放，抗過敏、促進肝功能與血小板凝集、以及降血脂等作用。有研究指出於液態培養時添加牛樟樹的樹皮萃取物或木削有利於牛樟芝的生長，是由於牛樟樹含有豐富的精油，且於先前研究中發現，液態培養時紅光光照及添加紅柑精油有助於樟芝三萜類的提升，因此，本研究選用三種皆屬於樟科樟屬的木材精油（牛樟精油、樟木精油、芳樟木精油）進行實驗，且經文獻證實，三種木材精油成份中以α-松油醇、芳樟醇以及樟腦等成份含量較多 [67] [68] [69]，亦有文獻指出α-松油醇以及芳樟醇有促進樟芝菌絲體生長的效果。所以初步先探討萜醇類對牛樟芝生長之影響，再進行添加牛樟精油、樟木精油、芳樟木精油，探討三種木材精油於不同濃度下對牛樟芝生成菌絲體及三萜類的影響。之後利用GC分析市售木材精油其成份及其成份的含量。最後，再進行於固定紅光光源的照射下，木材精油的添加對牛樟之生產三萜類之影響。 (1)添加單醇類或木材精油，雖然都對牛樟芝菌絲體的生長會產生抑制的現象，但卻可以提升三萜類化合物的產量。 (2)在萜醇類中，以添加0.05 mL/100 mL α-松油醇時，可以促進三萜類化合物產量達47.18 mg/g DW。 (3)在三種木材精油中，以添加0.1 mL/100 mL牛樟精油時，可以促進三萜類化合物產量達45.24 mg/g DW。 (4) 利用GC分析市售牛樟精油的成份，發現主要成份含有α-松油醇與芳樟醇。 (5) 添加木材精油會抑制菌絲體的生長，但若搭配紅光照射培養可以減緩菌絲體因添加精油而產生的抑制現象。 (6) 添加適量的牛樟精油精油可以有效提升三萜類化合物的產量，搭配紅光照射培養其三萜產量可以大幅提升。 ;Antrodia cinnamomea (AC) has attracted much attention recently due to its pharmacological benefits, especially for anticancer, and immunomodulation effects. Triterpenoids are the major bioactive compounds present in AC, and responsible for cancer curing ability. However, low triterpenoids content is observed in submerged culture. The aim of this research was to investigate the effect of addition of wood essential oil to the shake flask cultures of A. cinnamoea growing under the influence of red light for enhancing triterpenoids production. The results demonstrated that xylose can replace glucose as the carbon source. Although addition of wood essential oil increased the triterpenoids production, the biomass decreased significantly. Adding Cinnamomum kanehirai essential oil 0.1 mL/100 mL and irradiated with red light intensity 300 lux enhanced both biomass and triterpenoid production. The triterpenoid production increased to 57.09 mg/g DW in shake flask cultures. Results indicated that the red light alleviated the inhibitory effect of wood essential oil to mycelia.
Appears in Collections:	[National Central University Department of Chemical & Materials Engineering] Electronic Thesis & Dissertation

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