;Chlorophenols (CPs) pollution of the soil has become a major concern. The pollution of soil has been treated using physical and chemical processes that have proven to be much more expensive. By changing the traditional methodology, biodegradation has been used as a new methodology in which enzymes are used (peroxidase and laccase) to promote oxidative-coupling reaction between recalcitrant organic pollutants (e.g. 2,4-DCP, 2,4,6-TCP, 2,3,4,5-TeCP, PCP) and soil organic matter (SOM), which produce stable incorporated polymers.
In this study, the soils from Shamao Mountain, Changhua topsoil, Linkou and Taichung were collected and studied. The soil organic matter (SOMs) was determined. The SOMs and reaction time will affect enzyme activity in the environment. 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and catalyzing in aqueous medium are the optimizations to find the best activity reaction conditions with enzyme. Dissociated enzymes on the oxidative-coupling reaction in the difference phase (water and soil) will be discussed. The presence of microorganisms and organic matter in the soil as a coupling product , can promote the coupling reaction. The chlorine monomer (CPs in the soil) can be transfered into dimers, trimers of macromolecular products, which are stable incorporated polymer.When the content of organic matter is higher, the residual concentration of chlorophenols are also higher. Additionally, the moisture(%) of soil favors for oxidative-coupling reaction, and the effect of removal of chlorophenol is as follows; 100%>60%>30%. Relative quantitiy of coupling products is different in sterilization and unsterilization. When samples 2,4-DCP and 2,4,6-TCP of Taichung.; Shamao Mountain are catalyzed by laccase, their products which molecular weight were the same (339.1m/z). The oxidative-coupling reaction in soil is complicated so the trends can’t be correlated. Taking advantage of enough activity, enzyme can reduce chlorophenols and produce lots of coupling products in the same time. The effect of catalysts to chlorophenols is observed as that peroxidase showed better activity than laccase. Moerover, if numbers of chlorine in chlorophenols is higher, it will be more difficulty to remove.