English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 61345/61345 (100%)
造訪人次 : 16218326      線上人數 : 198
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋


    請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/72880


    題名: 跳躍子flea插入let-7 complex基因座可能導致mir-100之低表現量,進而造成果蠅存活率降低和發育遲緩;Hypo-expression of mir-100, reduced survival rate, and delayed development in Drosophila melanogaster may result from the insertion of flea transposon in let-7 complex locus
    作者: 林伯駿;Lin, Bo-Chun
    貢獻者: 生命科學系
    關鍵詞: miRNA;cluster;mir-100;let-7 complex;果蠅發育;microRNAs;miRNA cluster;mir-100;let-7 Complex;Drosophila development
    日期: 2017-01-20
    上傳時間: 2017-05-05 17:12:59 (UTC+8)
    出版者: 國立中央大學
    摘要: 跳躍子flea插入let-7 complex 基因座可能導致mir-100之低表現量,進而造成果蠅存活率降低和發育遲緩

    摘要

      miRNA是小片段的RNA分子,約含21至23個鹼基,可藉由與mRNA的互補配對,抑制蛋白質轉譯。而miRNA cluster,可藉由poly-cistronic轉錄方式使miRNA表現。在let-7 cluster裡包含miR-100、let-7、miR-125這三個miRNA,這個cluster在前人的研究中被證實會在果蠅幼蟲轉變成蛹時表現。但是,一個以microarray的方式偵測miRNA表現的研究中,發現Canton S (CS-UCI)品系中miR-100在前蛹期表現量較其它miRNA或其它品系miR-100表現量較低之現象。本研究以該研究所使用之品系為對象,並以Oregon R (OR-UCI)和得自於長庚大學皮海薇老師實驗室的Canton S (CS-PI)探討miR-100表現量異於同cluster之其他miRNA的原因。經由一系列PCR實驗,發現在CS-UCI的mir-100與let-7區間有一段插入的片段,定序後進行BLAST序列比對,發現這個插入片段有98%的序列與flea跳躍因子相似,因此推測flea可能造成miR-100的低表現量。在不同溫度的蛹存活率測試中,觀察CS-UCI的蛹存活率,於高溫環境下顯著較低,因此推測miR-100可能具有影響蛹於高溫環境中耐受力的功能,這一個獨特的現象,讓我們可以了解miR-100在果蠅生長中扮演的角色。

    關鍵詞:miRNA、cluster、mir-100、let-7 complex、果蠅發育
    ;Hypo-expression of mir-100, reduced survival rate, and delayed development in Drosophila melanogaster may result from the insertion of flea transposon in let-7 complex locus

    ABSTRACT

      MicroRNAs (miRNAs) are 21~23nt long RNAs, which regulate gene expression at post-transcriptional level by inhibiting the translation of target mRNAs through complementary pairing. Clustered miRNAs, such as let-7 cluster, are suggested to exhibit coordinate expression via a poly-cistronic transcription or inhibit translation. Let-7 cluster, consisting of miR-100, let-7, and miR-125, are increasingly expressed during larva-to-pupa transition in Drosophila melanogaster. However, the expression level of miR-100 was show to be lower than those of let-7 and miR-125 in Canton S strain as well as those of miR-100 in other strains in previous studies. In this study, we investigated the cause and the effects of low expression level of miR-100 in Canton S strain from UCI (CS-UCI) by using Oregon R (OR) strain and Canton S strains from Dr. Hai-wei Pi’s lab (CS-PI) as control strains. In qRT-PCR experiments, the expression level of miR-100 was confirmed to be low in CS-UCI but high in CS-PI and OR. A series of PCR experiments in genomic DNA revealed the presence of a flea element between mir-100 and let-7 in CS-UCI but not in CS-PI, suggesting the lower expression level of miR-100 might result from this insertion. Base on our preliminary tests, CS-UCI pupae showed significantly lower survival rate at 30℃ than the other two strains, suggesting miR-100 might involve in buffering heat stress during pupal development. The detail molecular mechanism on low expression level of miR-100 and its phenotypic effects in Drosophila development require further investigation.

    Key words: microRNAs、miRNA cluster、mir-100、let-7 Complex、Drosophila development
    顯示於類別:[生命科學研究所 ] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML65檢視/開啟


    在NCUIR中所有的資料項目都受到原著作權保護.

    社群 sharing

    ::: Copyright © National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋  - 隱私權政策聲明