以上皮細胞間質化與增生相關功能來描述癌症幹細胞之基因型;Genotypes of cancer stem cells characterized by epithelial-to-mesenchymal transition and proliferation related functions

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题名:	以上皮細胞間質化與增生相關功能來描述癌症幹細胞之基因型;Genotypes of cancer stem cells characterized by epithelial-to-mesenchymal transition and proliferation related functions
作者:	許爵麟;Hsu, Chueh-Lin
贡献者:	系統生物與生物資訊研究所
关键词:	癌症幹細胞;上皮細胞間質化;增生;cancer stem cell;epithelial-mesenchymal transition;EMT;proliferation;GSEA
日期:	2017-01-23
上传时间:	2017-05-05 17:18:09 (UTC+8)
出版者:	國立中央大學
摘要:	癌幹細胞或稱為具有幹細胞特性的癌細胞，通常表現耐藥性，以及高效率誘導癌症的能力。在過去幾年，公共資料庫收集的全基因組表現量數據，已有癌幹細胞分子與功能特徵的相關研究，這些材料足夠提供進一步的分析。在這裡，本研究使用公開的癌幹細胞全基因組基因表現量數據集，利用主成分分析篩選出十四組癌幹細胞組和四組控制組的高品質數據集。使用來自分子簽名資料庫的6,002個基因分子簽名，分析這十八組數據集。癌幹細胞數據集在本研究被區分為三種基因型。第一型由神經膠質瘤癌幹細胞主導，明顯強化增生相關功能，同時抑制上皮細胞間質化相關功能。第二型皆為乳癌幹細胞組成，顯著增強上皮細胞間質化相關功能，但無增生相關功能的反應。第三型由卵巢癌、前列腺癌、大腸癌的癌幹細胞所組成，有同時顯著抑制增生與上皮細胞間質化相關功能的錯雜現象。;Cancer stem cells (CSCs), or cancer cells with stem cell-like properties, generally exhibit drug resistance and have highly potent cancer inducing capabilities. Genome-wide expression data collected at public repositories over the last few years provide excellent material for studies that can lead to insights concerning the molecular and functional characteristics of CSCs. Here, we conducted functional genomic studies of CSC based on fourteen PCA-screened high quality public CSC whole genome gene expression datasets and, as control, four high quality non-stem-like cancer cell and non-cancerous stem cell datasets from the Gene Expression Omnibus database. A total of 6,002 molecular signatures were taken from the Molecular Signatures Database and used to characterize the datasets, which, under two-way hierarchical clustering, formed three genotypes. Type 1, consisting of mainly glia CSCs, had significantly enhanced proliferation, and significantly suppressed epithelial-mesenchymal transition (EMT), related functions. Type 2, mainly breast CSCs, had significantly enhanced EMT, but not proliferation, related functions. Type 3, composed of ovarian, prostate, and colon CSCs, had significantly suppressed proliferation related functions and mixed expressions on EMT related functions.
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