癌症目前在全世界死亡之首，其特徵癌細胞不受控制的繁殖，藉著血液、淋巴系統的循環，散播到身體其他部位，形成癌症的轉移。目前治療方法有腫瘤切除術、化學治療及放射性治療等治療方式，近年來研究發現機械力刺激細胞會影響細胞的生長因子，希望以機械力刺激癌細胞可以找到治療的機制，而微流體晶片進行生物檢測或分析具有降低人工操作的實驗誤差、自動化控制系統穩定性、降低耗能及樣品用量、時間等優點。 本研究以微流體晶片作為進行剪應力、正向應力刺激癌細胞的裝置，此裝置以設計出多項不同剪應力、正向應力刺激癌細胞，結構可將其區分成上氣閥層與下層流道層的剪、正向應力實驗晶片，氣閥層以負光阻製作出高深寬比的微結構，流道層以正光組製作出圓角的微結構，圓角有利於閥門元件能夠緊密關閉流道，利用螢光染液證實閥門的緊密度，先將癌細胞種植於下層的微流道內部腔體進行多重應力刺激系統的整合在微流體晶片上。再利用Lab view程式進行自動化控制氣閥門，及正向力氣室配合不同的時間和壓力進行刺激。 ;Cancer is currently the world′s leading cause of death, in recent years, research shows that mechanical stress are also found to have some effects to the cells . The side effects by chemo or radiation therapy may be reduced by the physical treatment assistance. We using microfluidic chip to physically and chemically stimulate cancer cells to study the mechanical stress effects to the cancer cells. Microfluidic chip for bio-detection or analysis has the advantages of reducing the manual error, automatic control system stability, reducing energy consumption and sample consumption, time, etc.
In this study, microfluidic chips were used as a device for shear stress and positive stress stimulation of cancer cells. The device to design a number of different shear stress, positive stress to stimulate cancer cells. The structure can be divided into the upper valve layer and the lower layer of the flow channel shear, positive stress test chip. The valve layer to negative light resistance to make a high aspect ratio of the microstructure. The flow path layer is made of round corners by the positive light group. So cancer cells are implanted in the lower chamber of the microchannel cavity for the integration of multiple stress stimuli on microfluidic chips, and then use the Lab view program to automatically control the gas valve, and the positive force chamber with different time and pressure to stimulate.
