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    Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/74087

    Title: 三硝基甲苯之毒理機制及生物降解暨多氯乙烯汙染模場生物整治
    Authors: 廖弘玉;Liao, Hung-Yu
    Contributors: 生命科學系
    Keywords: 生物降解;Biodegradation
    Date: 2017-09-21
    Issue Date: 2017-10-27 13:07:53 (UTC+8)
    Publisher: 國立中央大學
    Abstract: 三硝基甲苯常被使用在軍事或名間爆破用途,已經有許多研究證明三硝基甲苯會殘留在土地或地下水中,由於三硝基甲苯具有毒性,而其毒性機制尚未澄清,本研究第一部分,使用人類肝癌細胞HepG2和Hep3B,證明三硝基甲苯會誘導自由基產生,造成DNA的損傷,如果長期有此壓力存在可能導致癌症,進一步我們發現在高濃度的三硝基甲苯培養條件下會導致細胞內質網壓力及粒線體的破壞,造成細胞死亡;第二部分,由於上述我們證實三硝基甲苯對細胞會造成毒性,因此清除環境中三硝基甲苯的污染刻不容緩,我們於台灣南部受三硝基甲苯汙染之廠址篩選出一株可降解三硝基甲苯之本土菌株Citrobacter sp.,我們發現Citrobacter sp. 可以將三硝基甲苯轉化成4-amino-2,6-dinitrotoluene (4-ADNT)和2-amino-4,6-dinitrotoluene (2-ADNT),並且我們利用轉錄體學結和蛋白質體學調查Citrobacter sp.在降解三硝基甲苯過程中的分子機制,發現在降解過程中中心代謝機制受到抑制,並且抵抗環境壓力相關基因(例如: chaperones, transport-related proteins, and membrane proteins)表現量增加,我們推測這些基因與Citrobacter sp. 的去毒性有關,最後我們純化降解三硝基甲苯的關鍵蛋白NemA,證實此蛋白質確實為降解三硝基甲苯之關鍵酵素;第三部分,我們將整治研究實際於一多氯乙烯污染場址施作,多氯乙烯是一種難以用化學或物理法去除的環境汙染物,我們於台南永康區一處多氯乙烯汙染模場,添加slow polycolloid releasing substrate (SPRS),進行生物刺激法整治並在整治過程中利用次世代定序取代傳統變性析度凝膠電泳分析調查環境菌相變化,結果證實SPRS確實可刺激降解多氯乙烯菌株生長並增加降解。;There have been several reports of widespread contamination of soil and groundwater resulting from explosives, such as 2,4,6- trinitrotoluene (TNT). The accumulation of TNT also presents potentially hazardous effects to both humans and animals. In chapter I, we examined TNT-induced apoptosis via ROS dependent mitochondrial dysfunction and ER stress in HepG2 and Hep3B cells. Chapter II, the bacterial strain Citrobacter sp. was isolated from 2,4,6-trinitrotoluene (TNT) contaminated soil. We investigated the transcriptomic and proteomic responses of Citrobacter sp. to TNT by comparing profiles at 0 h and 12 h to understand how Citrobacter sp. can survive and transform under TNT stress. Chapter III, Tetrachloroethene (PCE) and trichloroethene (TCE) are pollutants found in large quantities in industrial areas around the world. The slow polycolloid releasing substrate (SPRS) has been developed to continuously provide biodegradable substrates for the enhancement of TCE reductive dechlorination. We investigated microbial groundwater community before and after SPRS addition and proved that can be stimulated the dechlorinating.
    Appears in Collections:[生命科學研究所 ] 博碩士論文

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