長DNA的Janus粒子; DNA-Grafted Janus Particles

Please use this identifier to cite or link to this item: http://ir.lib.ncu.edu.tw/handle/987654321/7544

Title:	長DNA的Janus粒子;DNA-Grafted Janus Particles
Authors:	薛京;Ching Hsueh
Contributors:	物理研究所
Keywords:	膠體粒子;Janus particles;DNA
Date:	2008-06-13
Issue Date:	2009-09-22 10:59:42 (UTC+8)
Publisher:	國立中央大學圖書館
Abstract:	我們發展了一種新興的材料—具有”頭”與”尾”的膠體粒子。所謂”頭”，是由一種雙重化學性質的粒子作為基礎—稱作Janus 粒子; 而”尾”則是由lambda-DNA 粘在這種粒子的半面所構成。我們以兩種方法來做Janus 粒子：石臘乳膠固定法及渡金法。另外我們也測試了三種不同的生物化學鍵結將lambda-DNA 長在Janus 粒子上：生物素-卵白素鍵結、胺-乙醛鍵結、以及金-硫醇鍵結。經過結合及比較以上的方法，我們認為要做出這種”頭尾”結合的粒子用結合渡金的Janus 微米粒子與硫醇-DNA 是一個有效的方法。 We developed novel colloidal particles which are composed of a head and tails. The “head” is based on Janus microspheres, amphiphlic particles with two different chemically modified hemispheres. The “tails”are attached by grafting l-DNA onto one of the hemisphere. Both wax-emulsion and gold-evaporation methods are used to generate Janus microspheres. We also test three different biochemical protocols to graft DNA onto silica surface: streptavidin-biotin binding, amine-aldehyde binding and thiol-gold binding. We compared all the methods and found that combining gold-evaporated Janus microspheres with thiol-DNA is an efficient way to fabricate the new class colloidal particles with head-and-tail structures.
Appears in Collections:	[Graduate Institute of Physics] Electronic Thesis & Dissertation

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