中大機構典藏-NCU Institutional Repository-提供博碩士論文、考古題、期刊論文、研究計畫等下載:Item 987654321/76767
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 78728/78728 (100%)
造访人次 : 33566727      在线人数 : 583
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.lib.ncu.edu.tw/handle/987654321/76767


    题名: 蘇雲金芽孢桿菌含有兩種Prolyl-tRNA synthetase基因;Bacillus thuringiensis possesses two prolyl-tRNA synthetase genes of distinct origins
    作者: 阮越康;Khang, Nguyen Viet
    贡献者: 生命科學系
    关键词: 蘇雲金芽孢桿菌;基因水平轉移;欖樹;Prolyl-tRNA synthetase;Horizontal gene transfer;Olive
    日期: 2018-07-16
    上传时间: 2018-08-31 11:36:58 (UTC+8)
    出版者: 國立中央大學
    摘要: Prolyl-tRNA synthetase (ProRS)是一種第二類型tRNA合成酶,按照序列分析兩種不同構造的ProRS存在於不同物種中:原核型與真核型。多數細菌只含有一種原核型ProRS基因,但蘇雲金芽孢桿菌含有兩種ProRS基因:proS1與proS2。令人驚奇的是,這兩種基因擁有不同的來源,且proS1的表現量遠大於proS2。根據同源tRNAPro的辨識部位,proS1應該是構造性基因,但proS2的功能則未知。這個多出來的proS2基因猜測是來自於基因水平轉移 (HGT),其所解碼的蛋白質可能在逆境中拯救proS1功能或參與含proline抗生素的合成。至於蘇雲金芽孢桿菌是如何將proline接到其對應tRNA上的機制,我們尚未能做完整解釋。ProRS的親源演化分析告訴我們,蘇雲金芽孢桿菌ProRS2和植物ProRS2有很高的序列同源性。另外,這細菌被發現和橄欖樹 (Olea europaea)有共生關係,這個發現暗示proS2基因可能是為了生存必須而從橄欖樹水平轉移過來的可能性。探討兩物種之間的生物相剋相生關係可以讓我們更加了解生物水平轉移的機制,也能對於ProRS的抑制機制獲得進一步的解釋。;Prolyl-tRNA synthetase (ProRS) is a class II synthetase which, according to sequence analysis, occurs in various organisms with one of two distinct structural architectures: prokaryotic-like and eukaryotic-like. While most of bacteria contain only one prokaryotic-like ProRS gene, Bacillus thuringiensis possesses two ProRS genes: proS1 and proS2. Intriguingly, both genes have different origins in which the expression of proS1 is much higher than that of proS2. Based on cognate tRNAPro identity elements, proS1 is supposed to be constitutive, whereas the function of proS2 remains unclear. The additional proS2 gene is hypothesized to be acquired by horizontal gene transfer (HGT) whose encoded protein (ProRS2) probably plays essential roles in rescuing the function of proS1 during stress conditions, participating in the biosynthetic pathways of antibiotics containing proline moiety, maintaining faithful translation. The question of how B. thuringiensis ProRS2 charges proline to its cognate tRNA remains to be characterized. The phylogenetic analysis of ProRS sequences showed that B. thuringiensis ProRS2 is well-aligned with plant ProRSs. The bacterium, additionally, can live symbiotically with olive tree (Olea europaea), raising a possibility that proS2 was transferred horizontally from the host tree during evolution course due to the necessity for survival. Understanding the allelopathic relationship between the two species could gain insight not only into the mechanism of HGT, but also into the inhibition of ProRSs.
    显示于类别:[生命科學研究所 ] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML213检视/开启


    在NCUIR中所有的数据项都受到原著作权保护.

    社群 sharing

    ::: Copyright National Central University. | 國立中央大學圖書館版權所有 | 收藏本站 | 設為首頁 | 最佳瀏覽畫面: 1024*768 | 建站日期:8-24-2009 :::
    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 隱私權政策聲明