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    題名: 皮膚微生物組體之產業運用與發展( II );The Skin Microbiome for Industrial Application and Development( II )
    作者: 黃俊銘
    貢獻者: 國立中央大學生醫科學與工程學系
    關鍵詞: 痤瘡;微生物組體;痤瘡桿菌;益生菌;Acne vulgaris;Microbiome;P. acnes;Probiotic bacteria
    日期: 2018-12-19
    上傳時間: 2018-12-20 11:29:27 (UTC+8)
    出版者: 科技部
    摘要: 人體益生菌可抵抗病原並維持微生物組體 (microbiome) 平衡,無選擇性的抗生素可摧毀這些益生菌。腸胃益生菌可製成優格保護消化道健康。我們團隊已發表很多論文第一次顯示用皮膚益生菌來治療青春痘。我們假設這些皮膚益生菌可用來調整失衡的青春痘微生物組體。我們的結果顯示皮膚表皮葡萄球菌 (S.epidermidis) 可藉甘油發酵,並產生短鏈脂肪酸 (SCFAs) 來抑制痤瘡桿菌 (P.acnes) 的過度生長。雖然我們證實了表皮葡萄球菌是一種皮膚益生菌,然可此菌也與醫院感染有關。因此本計畫將鑑定一株較安全的皮膚益生菌用來抑制痤瘡桿菌所以起的發炎。我們計畫與葡萄王公司合作開發青春痘益生菌。目標一,我們將從皮膚微生物組體中分離一種新的益生菌,並調查此益生菌是否可普遍性抑制不同痤瘡桿菌株。目標二, 我們將使用NMR 分析發酵液中的短鏈脂肪酸,並用老鼠的實驗篩選出一個最有活性短鏈脂肪酸以抑制痤瘡桿菌的生長,目標三,我們將研究此益生菌是否影響其他皮膚菌的生長,並檢查其對皮膚細胞的毒性。除了優格,酒與醋皆為利用微生物發酵之產品。每一項發酵產品可帶來每年超過一億美元的利潤。我們已在中央大學建立了一個皮膚益生菌銀行。我們將從此銀行的皮膚益生菌中鑑定出一株最安全且在體內外皆有效的青春痘益生菌。如果計畫成功,至少三樣產品包括皮膚益生菌,甘油益生元及短鏈脂肪酸將被開發成青春痘益生菌。 ;Antibiotics without selectivity may destroy the friendly microbes in the human microbiomethat helps fight pathogens and maintain homeostasis of microbiome. The fermentation product(yogurt) of friendly bacteria in human intestine promotes a healthy digestive system. With severalpublications, our group has for the first time demonstrated a skin probiotic approach for treatmentof acne vulgaris. We hypothesize that dysbiosis in the acne microbiome can be re-balance by theskin probiotic bacteria. Results in our previous publication indicated that Staphylococcusepidermidis (S. epidermidis), a skin bacterium, can fermentatively metabolize the glycerol to shortchainfatty acids (SCFAs) which suppress the growth of Propionibacterium acnes (P. acnes), anopportunistic bacterium highly associated with acne vulgaris. Although S. epidermidis hasfermentation activity, the bacteria represent one of causative agents involved with infections ofmany types of indwelling medical devices in hospital. Thus, we are here seeking a S. epidermidisalternative bacterium which can effectively rein in the over-growth of P. acnes as well as mitigatethe P. acnes-induced inflammation.In this collaborative proposal, we will team up with Grape-King Inc. at Taoyuan, Taiwan todevelop acne probiotics. The acne probiotics will be generated by fermentation of skinmicroorganisms in glycerol. In Specific Aim 1, we will identify the probiotic microorganisms fromthe human skin microbiome for the development of acne probiotics, and investigate the inhibitoryeffects of acne probiotics against various clinical P. acnes strains. In Specific Aim 2, we will profilethe SCFAs in the ferment metabolites of probiotic microorganisms using NMR spectrometers,select the most potent SCFA as an anti-P. acnes agent, and evaluate the bactericidal activities ofacne probiotics against P. acnes in vivo. In Specific Aim 3, we will assess the possible perturbationeffects of acne probiotics on skin commensals, and examine the cytotoxicities of acne probiotics.Besides yogurt, microbial fermentation has been widely employed in the development ofvarious products including wine and vinegar. Each fermentation industry can get annual profits ofover $100 million. A potent microorganism strain that can interfere with the growth of P. acnes viafermentation will be identified for a Skin Microbiome Bank established at PI’s lab at NationalCentral University for development of acne probiotics. In this two-year proposal, we will validatethe anti-P. acnes efficacy of probiotic bacteria and SCFAs in vitro and in vivo. When successful,an acne probiotic containing at least three products [probiotic bacteria, glycerol as a fermentationsubstrate, and SCFAs) will be obtained for treatment of acne vulgaris.
    關聯: 財團法人國家實驗研究院科技政策研究與資訊中心
    顯示於類別:[生醫科學與工程學系] 研究計畫

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