反向虛擬物鏡(opposite virtual objective，OV-Obj)為我們首創的新式動態光學技術，是目前國際文獻 僅見唯一能夠在厚生物體產生背向聚焦光源的技術， 本計畫為了結合OV-Obj 與數位波前調製 技術，使OV-Obj 能夠被實用於螢光顯微術、諧波訊號顯微術與及4-pi 相關應用，將架設具有持續 能量放大能力之數位光學相位共軛 (Digital optical phase conjugator, DOPC)系統，以利用數位波前調 製技術產生夠強的反向聚焦訊號。研究逐年分成三階段：第一年度使用一組CMOS 影像偵測器與空 間光調製器取代光折變晶體，建立讀取光與參考光彼此獨立的DOPC，使用小貓自泵浦相位共軛器 (Kitty-type self-pumped phase conjugator，Kitty-SPPCM)產生6 軸校正訊號，並使用六軸電控平台進行 自動校正，再進一步調整架構使DOPC 能夠提高持續放大訊號。第二年度利用DOPC 建構生物組織 中的時間微分粒子追蹤聚焦系統，產生物體內自發光源，此種光源由於不受組織相位擾動影響，其 聚焦品質較外部聚焦光源更為優良。第三年度建立結合DOPC 與OV-Obj 的4-pi 顯微鏡，並掃描實 物進行驗證，最後建立DOPC 的光學模型，以做為進一步研發動態光學之關鍵技術。 ;Opposite virtual objective (OV-Obj) as our innovation of new dynamic optical technique, is the only technique that can produce inverse focusing point inside thick tissue, to the best our knowledge. In order to combine OV-Obj and digital wave front modulation technique, and to apply OV-Obj in the field of fluorescent microscopy, harmonic-signal microscopy, and 4-pi related application, this project will build a continuous-amplified Digital optical phase conjugator. It use the digital wave front modulation technique to produce inverse focusing signal with enough power. The study is divided into three phase year by year: In the first year, we use the set of a CMOS image sensor and a spatial light modulator to replace the birefrigent crystal, and thus build up a DOPC system with reading beam and reference beam independent to each other. The Kitty-type self-pumped phase conjugator (Kitty-SPPCM) is applied to produce 6-dimension alignment signal. Automatic alignment is implemented by a 6-axis electrical stage. The architecture is further improved to produce high-gain continuous-amplified DOPC. In the second year, we build up the time-differential particle-tracking focusing system, and produce light source inside the tissue. Because the time-differential technique is invariant to the phase disturbance of the tissue, this kind of light source is superior to the epi focusing light source. In the third year, we build up the 4-pi microscope that composed of DOPC and OV-Obj. The idea will be demonstrated by scanning some samples. Finally, we build up the optical model of DOPC, which will be the key technique for further research of dynamic optics.
