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|Title: ||綠茶唲茶素對HIB1B棕色前脂肪細胞生長的影響;Effect of green tea catechins on the growth of HIB1B brown preadipocytes|
|Authors: ||鄭喻心;Cheng, Yu-Hsin|
|Keywords: ||HIB1B 棕色前脂肪細胞;綠茶唲茶素;生長;HIB1B brown preadipocytes;green tea catechins;growth|
|Issue Date: ||2019-04-02 14:21:40 (UTC+8)|
Green tea catechins (GTCs) are a type of natural phenol and antioxidants. They are associated with a variety of health benefits, including the maintenance of cardiovascular health, the reduction of cancer risk, and weight loss. In particular, epigallocatechin gallate (EGCG) was found to reduce the fat synthesis, adipogenic differentiation and preadipocyte growth, while it induced weight loss, lipolysis, and thermogenesis. Although GTCs selectively inhibited the growth of 3T3-L1 white preadipocytes, the exact mechanism of their actions on brown preadipocyte growth is still unknown. Using HIB1B brown preadipocytes, we found that GTCs decreased the growth of brown preadipocytes. EGCG time- or dose-dependently decreased levels of the cell number, BrdU incorporation, and cell viability. EGCG was more effective in inhibiting growth of brown preadipocytes than other green tea catechins, such as epicatechin, epicatechin gallate, and epigallocatechin. This suggests the structure type-dependent effect of green tea. In addition, the potency of EGCG to reduce the growth on brown preadipocytes was greater than that on white preadipocytes, as indicated by changes in cell number. Further Western blotting analysis indicated that EGCG tended to time-dependently increase the phosphorylation of ERKs and AMPK proteins. These observations were different from those reported for EGCG inhibition of ERKs phosphorylation in 3T3-L1 white preadipocytes. These results suggest the ERK-dependent effect of EGCG on preadipocyte mitogenesis, as well as the possible different role of ERKs proteins in mediating the effect of EGCG on the growth between brown and white preadipocytes.
|Appears in Collections:||[生命科學研究所 ] 博碩士論文|
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