台灣沉香樹相關的內生菌;Endophytic microorganisms associated with agarwood trees in Taiwan

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Title:	台灣沉香樹相關的內生菌;Endophytic microorganisms associated with agarwood trees in Taiwan
Authors:	李振嘉;Lee, Chen-Chia
Contributors:	生命科學系
Keywords:	沉香;Aquilaria sinensis;Agarwood;Endophytic fungi
Date:	2019-01-17
Issue Date:	2019-04-02 14:21:50 (UTC+8)
Publisher:	國立中央大學
Abstract:	沉香為一種昂貴的木材，應用於薰香與香水產業，為 Aquilaria spp. 或 Gyrinops spp. (瑞香科)的樹木受到真菌感染並處於病理條件下形成的一種黑色油樹脂。為了探討台灣台東縣太麻里鄉之沉香相關的內生菌，我們以消毒過的生長錐與刀片分別採集活體木材(可能含有沉香木和健康的木材)以及樹齡20歲左右的 A. sinensis 之樹幹底部的死沉香木進行內生菌的分離。菌株根據菌落型態和分子鑑定來區別真菌和細菌物種。在2017年2月到2018年2月的四次採集中，總共分離了617個菌株，並鑑定出34種真菌物種與7種細菌物種。其中 Lasiodiplodia hormozganensis (34個菌株，占總菌株數的5.5%)在每次採集都有出現，大多分離於死沉香木(32株)。 Trichoderma lentiforme (236個菌株，占總菌株數的38.3%)出現於其中三次採集，大多分離於健康的木材(175株)。另外 Aspergillus fijiensis， Fusarium solani species 28 和 Phaeoacremonium rubrigenum 也在四次採集中出現了兩次。而在細菌方面，大多數分離於死沉香木(Paenibacillus lautus，41個菌株，占總菌株數的6.7%)。在感染實驗中，以 T. lentiforme 和 L. hormozganensis 作為病原菌感染兩歲大的 A. sinensis 。本研究利用內生真菌以人工誘導的方式，使 A. sinensis 產生沉香同時奠定基礎並促進台灣沉香產業的發展。;Agarwood is the most expensive wood product and used in the incense and perfume industry. It is a dark resinous wood that forms in Aquilaria and Gyrinops trees (Thymelaeaceae family) under pathological conditions, mainly fungal infection. In order to isolate endophytic microorganisms associated with agarwood at Taimali Township, Taitung County, Taiwan, samples were aseptically taken with an increment borer from living wood (containing presumably fresh agarwood and healthy wood), standardized borecores as well as cut off non-standardized agarwood from exposed old agarwood from living stems of a ca. 20-years-old plantation of A. sinensis. Microbial strains were classified to morphospecies and identified to species with specific molecular genetic markers depending on the systematic groups of fungi or bacteria. A total of 617 strains comprising 34 fungal and 7 bacterial species were identified from 4 collection events between February 2017 and February 2018. Strains of Lasiodiplodia hormozganensis (34 strains, 5.5% of total strains) were isolated at every collection event, 2 from discolored wood, 0 from non-agarwood and 32 from dead agarwood. Strains of Trichoderma lentiforme (236 strains, 38.3% of total strains) were isolated at three collections, 175 associated with discolored wood, 29 from non-agarwood and 32 from dead agarwood. Aspergillus fijiensis, Fusarium solani species 28 and Phaeoacremonium rubrigenum were isolated at two collection events. Bacteria (Paenibacillus lautus, 41 strains, 6.7% of total strains) were almost only isolated from dead agarwood. T. lentiforme and L. hormozganensis species identified as candidate pathogens were used in inoculation experiments in two-year-old A. sinensis. The study lays the foundation for the development of endophytic fungi for artificial infection methods in A. sinensis to induce agarwood and promote the agarwood industry in Taiwan.
Appears in Collections:	[Graduate Institute of Life Science] Electronic Thesis & Dissertation

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