非特異性蛋白質吸附是生物醫學應用中最重要的問題，例如植入式 醫療器械。此外，自組裝單層的降解受到廣泛關注，因為它被認為 是決定這些系統適用性的關鍵因素。研究中，發現很多對不同血液 成分具有高抗污性的穩定塗層材料，並且提出不同策略作為增強自 組裝單層的穩定性的解決方案。在這項工作中，我們合成了三種磺 基甜菜鹼基單體; （單硫醇）3 - [（11-巰基-十一烷基）-N，N-二 甲基 - 氨基] - 丙烷-1-磺酸“SB-硫醇”，（二硫化物）硫辛酸 - 磺基乙烷“SBSS”和（二硫醇）二氫脂質酸 - 磺基甜菜鹼“SB-二 硫醇”，並比較了它們的自組裝單層的構成，穩定性和抗吸附性。 將SBSS 還原成SB-二硫醇導致更高的覆蓋率，對不同蛋白質的抗吸 附性，更高的阻斷電子轉移能力和對解吸電壓掃描的有效穩定性， 如表面等離子體共振（SPR）形成測試所示，並進行蛋白質抗吸附性 試驗和電化學分析。儘管SB-二硫醇在潤濕性方面表現出與SB-硫醇 相似的能力，並且對不同蛋白質具有抗吸附性，但前者在”接觸角 分析”，”厚度分析”，”表面等離子體共振分析”和”電化學分 析”中所有的穩定性測試顯示出更好的特性。這項研究將使我們對 類似兩性離子自組裝單層與金和鄰近部分的相互作用有所了解，並 有望為未來的許多應用奠定基礎。;Non-specific protein adsorption is the most important problem in biomedical applications, such as implantable medical devices.1 In addition, the degradation of self-assembled monolayers has received a broad attention as it is regarded a key factor that determines the applicability of these systems into many applications. Many studies have been introduced to give stable coating materials with high fouling resistance against different blood components and different strategies have been proposed as a solution to enhance the stability of the self-assembled monolayers.2 In this work, we synthezied three sulfobetaine-based monomers; (monothiol) 3-[(11-mercapto-undecyl)-N,N-dimethyl-amino]-propane-1-sulfonic acid “SB-thiol“, (disulfide) lipoic acid-sulfobetiane “SBSS“ and (dithiol) dihydrolipoic acid-sulfobetaine “SB-dithiol“, and compared the formation, stability and fouling resistance of their self-assembled monolayers. The reduction of SBSS into SB-dithiol3 resulted in a higher coverage, a better fouling resistance against different proteins, a higher capability to block electron transfer and an effective stability against desorption voltage sweep as was shown by the Surface Plasmon Resonance (SPR) formation test, protein fouling resistance test and the electrochemical analysis respectively. Although SB-dithiol exhibited a similar behavior to SB-thiol in the wetting properties and the fouling resistance against different proteins, the former showed a privilege in all the stability tests “contact angle analysis”, “thickness analysis”, “Surface Plasmon Resonance analysis” and electrochemical analysis. This study will develop our understanding of the interactions of analogues zwitterionic SAMs with gold and adjacent moities, and it is expected to put the basis for many future applications.
