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|Title: ||硬脂基化的Indolicidin作為傳送質體去氧核 酸的非病毒載體;Stearylated Indolicidin as a nonviral vector for plasmid DNA delivery|
|Authors: ||沈筱容;Shen, Hsiao-Jung|
|Issue Date: ||2019-09-03 12:35:30 (UTC+8)|
|Abstract: ||細胞穿透肽（CPP）已經被廣泛研究作為用於基因傳送的非病毒載體。 Indolicidin（IL）是一種有潛力的細胞穿透肽，其富含陽離子精氨酸、賴氨酸和疏水性的色氨酸。雖然IL已被用作小分子核苷酸的載體，例如siRNA和寡脫氧核苷酸（ODN）的傳送，但它不能單獨遞送大分子質體DNA（pDNA）。雙親性分子其親疏水段鍊可自組裝成膠束或脂質體等微結構，藉此與細胞膜融合達到藥物輸送的效果。因此，本研究將IL的N或C末端進行硬脂基化，並分別將命名為sIL和ILs。|
;Cell-penetrating peptides (CPP) have been investigated as a non-viral vector for gene delivery. Indolicidin (IL) is a potential cell-penetrating peptide rich in cationic arginine/lysine and hydrophobic tryptophan. Although IL has been applied as a carrier for small nucleotides such as siRNA and oligodeoxynucleotide, it cannot solely deliver huge plasmid DNA (pDNA). Amphiphilic molecules can self-assemble as micelles or liposomes due to their hydrophilic/hydrophobic domains to promote their fusion with cell membranes for drug delivery. Therefore, we modified IL by stearylating its N and C terminals, and denoted them as sIL and ILs, respectively. The DLS analysis was applied to examine the size and surface charges of these stearylated peptides, suggesting that sIL and ILs were capable of self-assembling as multilamellar vesicles (MLVs) and inverted micelles, respectively. The DNA loading examination demonstrated that stearylation of peptides did not hinder their complexation with DNA. The competition experiments showed that self-assembled structure of stearylated peptides increased the stability of complexes. Because delivery efficiencies of CPPs highly depend on their interaction with cell membranes, calcein leakage was applied to evaluation their membrane perturbation ability. In contrast to sIL which demonstrated poor leakage due to its N terminal-stearylation, membrane perturbation ability of ILs was similr to that of unmodified IL. When these peptides were applied for gene delivery, the results of confocal microscopy and flow cytometry showed that only ILs promoted DNA internalization. The transfection results indicated that ILs successfully transfected HEK-293T cells, whereas sIL and IL demonstrated almost no transfection. Due to poor membrane perturbation ability and huge sizes of sIL/DNA complexes, sIL cannot promote DNA transportation. Although IL can directly complex with DNA with appropriate size and good loading efficiency, these complexes is not stable enough. Therefore, IL may adsorb to cell membrane during transfection to release DNA extracellularly. In contrast, ILs may force its N terminus to interact with cell membrane to promote perturbation. The self-assembly of inverted micelles may also stabilize ILs/DNA complexes. These properties explain the improvement effects of ILs on DNA delivery efficiency.
|Appears in Collections:||[化學工程與材料工程研究所] 博碩士論文|
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