近年來，科技的進步讓人類生活的水平提高，為了保持優良的生活品質，也帶動生物科技和醫療的發展，使生醫學門成為新興的熱門研究議題。為了能在生醫感測器上偵測到低濃度的稀疏單分子為最終目標，本研究在表面增強共振型拉曼散射效應的原理下，以低成本的金屬有機化學氣相沉積法在氧化鋅奈米柱上磊晶出在半導體上具有高折射率、高化學穩定性的氮化銦鎵量子井，形成金字塔狀的粗糙表面，在搭配惰性佳的金奈米顆粒，以螢光分子R6G為待測物，來分析此獨特的奈米二維結構基板在生醫感測上的應用。 透過R6G在拉曼光譜上的表現，反映出此獨特結構的巨大優勢，此奈米結構基板除了能有高達106的EF值，可偵測到10-12M的極低分子的濃度，有著大面積且分布均勻的熱點，更有低成本地製作，簡單方便地量測，穩定的增強性且輕薄小的體積等種種優勢，在生醫感測及單分子偵測上有極大的潛力。 ;In recent years, the advancement of science and technology has improved the level of human life.In order to maintain a good quality of life, it has also promoted the development of biotechnology and medical care,and made the biomedical science become a hot topic of research in the world. To get the reliable signals from sparse single molecules in low concentration is the final goal of biosensors. In order to achieve it, in this study, under the principle of surface-enhanced resonance raman scattering(SERRS), InGaN quantum wells with high refractive index and high chemical stability on the semiconductor are deposited on ZnO nanorod which are epitaxial grown in low-cost way by MOCVD and it has a rough and nanopyramid-like surface with Au nanoparticles. And the Rhodamine6G a kind of fluorescent molecules are used as the analyte to analyze the application and potential of this unique 2D nanostructured substrate in biosensing. The performance of R6G on the Raman spectrum reflects the enormous advantage of this unique structure. In addition to an EF value is high to 106 and limit of detection is down to 10-12 that is very low concentration of molecular, it also has a large area with the “hot spots” which are evenly distributed on surface, and low-cost of production, the simple and convenient of measurement, the stability of enhancement , the small, thin and light of the volume, there is great potential in biosensing and single molecule detection.
