動態干涉儀應用於量測活體微生物之光學常數;Measuring the optical constant of living microorganisms by a Linnik-type dynamic interferometer

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题名:	動態干涉儀應用於量測活體微生物之光學常數;Measuring the optical constant of living microorganisms by a Linnik-type dynamic interferometer
作者:	陳詣欽;Chen, Yi-Qin
贡献者:	光電科學與工程學系
关键词:	動態干涉儀;活體微生物檢測;Linnik-type dynamic interferometer;microorganism measurement
日期:	2019-07-17
上传时间:	2019-09-03 14:44:58 (UTC+8)
出版者:	國立中央大學
摘要:	近幾年來隨著癌症的發生人數不斷攀升，不少人投入研究癌細胞與正常細胞之間有何不同之處，其差異之處在於：細胞形狀、細胞核大小等，為了在不破壞樣品前提下，又能清楚分辨細胞核位置，因此利用細胞核與細胞質之間的折射率差異定位細胞核位置。目前檢測全場生物折射率的儀器大多為掃描式干涉儀，但此種干涉儀在檢測中易受環境干擾，需要一段時間才能求得折射率，因此本論文將提出解決環境干擾與快速求得折射率之方法。本實驗中以Linnik-type偏振式干涉儀結合4D Technology公司出產的攝影機當作實驗架構，有效降低環境的干擾，即使在光學桌不充氣的情況下，也能有效量測活體微生物。為了測試本實驗架構的可行性，首先檢測光學薄膜進行確認，其厚度誤差為0.03%，標準差為0.74nm，折射率誤差為0.24%，標準差為0.0032。微生物公認的細胞質折射率範圍在1.35至1.37之間，本實驗檢測出的微生物細胞質折射率在其範圍內。而為了達到即時檢測折射率的變化，本實驗亦利用演算法取得微生物的厚度，計算出其相位調制級數並輸入程式，即可即時算出整體活體微生物的折射率及厚度，達到即時動態量測效果。;In recent years, due to the number of people who are ill with cancer grow up, many people have studied the difference between cancer cells and normal cells. The differences between cancer cells and normal cells include cell shape, cell size, etc. In avoid to damaging the sample and the position of the nucleus can be clearly distinguished, the position of the nucleus is located by the difference in refractive index between the nucleus and the cytoplasm. At present, most of the instruments for detecting the whole field refractive index are scanning interferometers, and they are susceptible to environmental interference during the detection. It takes a while to obtain the refractive index. This paper will propose a method to solve the environmental interference and quickly obtain the refractive index. In this experiment, the Linnik-type polarization interferometer combined with the camera produced by 4D Technology Company is used as the experimental framework to effectively reduce the environmental interference, and the biological measurement can be effectively measured even when the optical table is not inflated. In order to test the feasibility of the experimental instrument, the optical film was first to be analyzed. The thickness error was 0.03%, the standard deviation was 0.74 nm, the refractive index error was 0.24%, and the standard deviation was 0.0032. The recognized cytoplasmic refractive index ranges from 1.35 to 1.37, and the microbial cytoplasmic refractive index detected in this experiment is within its range. In order to achieve the instantaneous detection of the change of refractive index, this experiment uses the algorithm to obtain the thickness of the microorganism, and calculates the phase modulation level. By inputting the phase modulation series into the program, the refractive index and thickness of the whole living microorganism can be calculated instantly.
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