本研究為了解焚化灰渣經碳酸鹽礦化此穩定化技術處理前後的危害特性變化，利用已商業套組化的Microtox生物毒性試驗法，量化灰渣經碳酸鹽礦化後在「生物層面」上的無害化成效。同時，本研究亦透過再現性、重複性及敏感度等指標評估Microtox應用在固體廢棄物相關研究的適用性，以了解灰渣溶出液毒性的來源及僅用化學分析是否已足夠做為固體廢棄物無害化的依據。檢測時發現溶出液樣品在上機分析前需進行pH值的調整，以排除因高鹼性所造成毒性高估的問題；此外，也觀察到並非所有的溶出液樣品對模式生物Vibrio fischeri所帶來的毒性效應皆可在15分鐘內達平衡，顯示後續的研究需留意此平衡時間上的判定，以免低估樣品真正的毒性效應。更值得注意的是，以Microtox檢測時發現灰渣穩定反應後樣品具有較高的毒性。但即使如此，以本研究的環境條件所得到的灰渣Microtox檢測數據皆呈現為第五級-無毒性。;In this study, to understand the changes of the hazard associated with the pre- and post-carbonation of the municipal solid waste incinerator (MSWI) residues (both bottom ash and fly ash) from the perspective of “biological effect”, the Microtox bioassay was adopted in this investigation. In the meantime, analysis of the reproducibility, repeatability, and sensitivity of the assay was performed. Results from this study may help determine the correlation between chemical and ecotoxicological characteristics of the MSWI residue leachates and also if the chemical analysis alone would be sufficient to be a basis for the regulation on the harmlessness of solid waste. Throughout the experiments, it was found that for Microtox assay, pH adjustment of the TCLP leachates was a necessitate in an effort to eliminate the interference resulting from elevated hydroxide levels of MSWI residues. Further, toxicity was not equilibrated in Microtox within the 15 min exposure suggested by the protocol, indicating that to avoid underestimation of sample toxicity, appropriate exposure periods should be cautioned. Most surprisingly, Microtox readings showed that toxicity increased after carbonation. Nonetheless, all the tested samples were classified as the 5th level material – which is non-toxic.
